|
| Status |
Public on Aug 03, 2010 |
| Title |
zebrafish_han_WT_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
hand2 wild-type sibling,19hpf
|
| Organism |
Danio rerio |
| Characteristics |
stage: 19 hpf
tissue: Whole embryo
|
| Growth protocol |
Embryos were collected, grown in blue water (60 mg/mL Instant Ocean and 0.025% Methylene blue) at 28.5 degrees Celcius and dechorionated prior to RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
3-9mg of total RNA from 10 to 25 embryos per sample were isolated using the RNeasy kit (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
ambion megascript labeling, with biotin labeled dNTP
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
According to manufacturer
|
| Data processing |
MAS5, overall intensity was targetted to 500
|
| |
|
| Submission date |
Aug 02, 2010 |
| Last update date |
Aug 02, 2010 |
| Contact name |
Zayra Viviana Garavito-Aguilar |
| E-mail(s) |
zgaravitoaguilar@ucsd.edu
|
| Fax |
(858)534-0555
|
| Organization name |
University of California, San Diego
|
| Department |
Division of Biological Sciences
|
| Lab |
Yelon Lab
|
| Street address |
9500 Gilman Drive #0347
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0347 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE23381 |
Transcription profile of zebrafish hand2 (hanS6 allele) mutant embryos at 19hpf |
|
