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Sample GSM5753 Query DataSets for GSM5753
Status Public on Apr 05, 2003
Title Spleen vs Sphingosine-1-phosphate Treated B cell 240min (set2)
Sample type RNA
 
Channel 1
Source name Spleen was labeled with Cyanine-3 (green).
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Sphingosine-1-phosphate Treated B cells were labeled with Cyanine-5 (red)
Organism Mus musculus
Extracted molecule total RNA
 
 
Description total RNA from mouse (male c57BL/6) spleen
total RNA from mouse (male c57BL/6) B cells treated with Sphingosine-1-phosphate for 240min
 
Submission date Apr 05, 2003
Last update date May 27, 2005
Contact name Sangdun Choi
E-mail(s) schoi@its.caltech.edu
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platform ID GPL260
Series (2)
GSE396 Spleen vs Sphingosine-1-phosphate Treated B cell
GSE2050 Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands

Data table header descriptions
ID_REF Feature number which correpond to the order of genes on the array
RATIO Raw Log10 Ratio
G_PROCESSED Green Processed signal, after dye normalization and background subtraction, used for computation of log ratio
R_PROCESSED Red Processed signal, after dye normalization and background subtraction, used for computation of log ratio
G_MEAN Green Channel Mean Intensity
R_MEAN Red Channel Mean Intensity
G_MEDIAN Green Channel Median Intensity
R_MEDIAN Red Channel Median Intensity
G_SD Green Channel Standard Deviation
R_SD Red Channel Standard Deviation
G_BG_MEAN Green Channel Mean Background Level
R_BG_MEAN Red Channel Mean Background Level
G_BG_MEDIAN Green Channel Median Background Level
R_BG_MEDIAN Red Channel Median Background Level
G_BG_SD Green Channel Background Standard Deviation
R_BG_SD Red Channel Background Standard Deviation
VALUE Processed Log2 Ratio after removing saturated spots, spot below 2.6XSD of background, and nonuniform spots.

Data table
ID_REF RATIO G_PROCESSED R_PROCESSED G_MEAN R_MEAN G_MEDIAN R_MEDIAN G_SD R_SD G_BG_MEAN R_BG_MEAN G_BG_MEDIAN R_BG_MEDIAN G_BG_SD R_BG_SD VALUE
1 -0.51549 33774.9 10306.3 65516.3 23327 65516 22804 0.620174 7037.33 652.577 1054.3 648 1047.5 29.4991 61.9203 0
2 -0.126539 128.827 96.265 690.154 1009.31 688.5 1007 38.5047 46.9832 631.987 1038.9 631 1040 18.5773 54.7826 0
3 -0.00565073 91.3223 90.1418 641.963 1037.85 632 1026 36.8129 79.0437 633.701 1040.81 631.5 1043 20.064 55.1776 0
4 -0.161526 117.919 81.2939 670.37 974.741 661 979 29.0691 36.9069 632.892 1037.51 631.5 1032 21.0246 56.4284 0
5 -0.0576252 89.2137 78.1279 631.24 1001 634 991 17.4267 70.6464 629.038 1040.39 627 1035 19.7314 52.9055 0
6 -0.20464 264.537 165.138 948 1078.08 924 1076 94.1293 83.1199 642.734 1017.43 641 1014 19.4699 56.139 0
7 0.042209 88.7593 97.8189 646.458 1082.79 649 1071.5 24.0561 67.8553 639.132 1057.39 640 1058 22.6162 59.0297 0
8 0.0375988 90.6455 98.8428 652.522 1098.91 647 1088 37.7514 80.9461 642.632 1073.34 639.5 1068.5 25.6199 65.9816 0.12490051
9 0.134503 2032.59 2770.47 4702.83 6380.72 4637 6372 249.557 398.247 643.539 1038.41 640 1033.5 22.5427 54.3487 0.446809295
10 0.641369 602.512 2638.36 1742.32 6327.28 1682 6310 319.987 1770.81 635.966 1050.93 633 1047.5 25.5509 61.0146 2.1305817
11 -0.0104451 89.1816 87.0623 634.448 1054.38 634 1063 24.7828 54.7647 629.6 1064.09 628 1058 21.1226 62.711 -0.034697871
12 0.023365 86.7985 91.5961 638.174 1097 645 1097 19.4554 35.1024 634.847 1088.29 632 1085 22.9655 64.5146 0.07761685
13 0.0119748 83.181 85.5065 633.385 1091.38 631.5 1084.5 20.1655 42.624 635.868 1096.89 636 1103 20.2322 62.3613 0.039779425
14 0.592748 441.523 1728.63 1389.56 4571.93 1425 4612 135.529 636.205 634.23 1101.49 633 1101 20.3537 61.2393 1.969066234
15 0.201974 1212.39 1930.25 2987.07 4848.48 1344 2202 2955.23 4669.29 648.645 1167.61 645 1163 25.7161 63.0924 0
16 0.311203 969.61 1985.18 2511.38 5082.46 1983 4196 1092.47 2162.53 670.533 1252.65 660 1227.5 34.9668 126.974 0
17 -0.402402 901.96 357.096 2178.24 1594.62 1891 1562 864.548 250.629 657.548 1166.51 653 1162 29.0193 90.6336 0
18 0.182704 184.164 280.485 846.304 1495.87 848 1479 27.4151 90.6584 648.682 1117.49 642 1110 28.4506 88.7868 0.606929551
19 0.399521 3777.13 9477.26 8271.86 20289 8459 21080 1526.11 3892.45 635.457 1055.61 631.5 1052 26.2553 69.7353 1.327180034
20 0.130704 3597.63 4860.95 7936.08 10603.6 7930 10560 889.04 1387.37 626.618 1000.64 621 991 23.4323 61.5672 0.43418929

Total number of rows: 16273

Table truncated, full table size 1976 Kbytes.




Supplementary data files not provided

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