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Sample GSM576 Query DataSets for GSM576
Status Public on Oct 11, 2001
Title BM_CD34-1b
Sample type RNA
 
Source name bone marrow flow-sorted CD34+ cells (>95% purity)
Organism Homo sapiens
Extracted molecule total RNA
 
Description Comparison of gene expression in CD34+ cells from bone marrow and G-CSF mobilized peripheral blood by high density oligonucleotide array analysis.
This sample represents a separate biotinylated cRNA preparation from the cells described in sample BM_CD34-1a (GSM575), hybridized to a different chip.
Sample hybridization parameters:
Background Avg: 469.14, Stdev: 13.94, Max: 490.4, Min: 449.5
Corner+ Avg:1378, Count:32
Corner- Avg:45744, Count:32
BF= SDT=94.5(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=17.08 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=94.5 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 TGT=1000 NF=1.000000 SF=1.383271 SFGene=All
 
Submission date Sep 26, 2001
Last update date May 27, 2005
Contact name Lynn Graf
E-mail(s) lgraf@fhcrc.org
Phone 206-667-4545
Fax 206-667-5978
URL http://none
Organization name Fred Hutchinson Cancer Research Center
Department Clinical Research Division
Lab Torok-Storb
Street address 1100 Fairview Ave. N. D1-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL80
Series (1)
GSE500 CD34+ cell analysis

Data table header descriptions
ID_REF
VALUE AverageDifference hybridization intensity
POSITIVE number of pairs with positive signal
NEGATIVE number of pairs with negative signal
PAIRS number of PerfectMatch and MisMatch oligo pairs specifying sequence
PAIRS_USED number of pairs used for analysis
PAIRS_IN_AVG trimmed probe pair set
POS_FRACTION #positive probe pairs/#of probe pairs used
LOG_AVG log average ratio (see Affymetrix literature for formula)
POS/NEG ratio of positive to negative pairs
ABS_CALL absolute call of Presence or Absence of gene transcript in sample

Data table
ID_REF VALUE POSITIVE NEGATIVE PAIRS PAIRS_USED PAIRS_IN_AVG POS_FRACTION LOG_AVG POS/NEG ABS_CALL
AFFX-BioB-5_at 22.7 6 2 20 20 18 0.3 0.66 3 A
AFFX-BioB-M_at 83.1 5 1 20 20 18 0.25 0.48 5 A
AFFX-BioB-3_at 391 13 2 20 20 18 0.65 2.47 6.5 P
AFFX-BioC-5_at 475.6 14 0 20 20 19 0.7 3.58 Undef P
AFFX-BioC-3_at 20.8 5 5 20 20 18 0.25 0 1 A
AFFX-BioDn-5_at 530.3 13 0 20 20 18 0.65 3.13 Undef P
AFFX-BioDn-3_at -426.4 7 7 20 20 20 0.35 0.24 1 A
AFFX-CreX-5_at 936.4 17 0 20 20 19 0.85 4.16 Undef P
AFFX-CreX-3_at 520.3 11 1 20 20 17 0.55 3 11 P
AFFX-BioB-5_st 2714.2 13 1 20 20 19 0.65 3.43 13 P
AFFX-BioB-M_st 133.6 9 3 20 20 17 0.45 0.98 3 M
AFFX-BioB-3_st 898.6 11 1 20 20 19 0.55 1.76 11 P
AFFX-BioC-5_st -590.2 4 7 20 20 18 0.2 -0.58 0.6 A
AFFX-BioC-3_st -580.1 8 3 20 20 19 0.4 0.9 2.7 A
AFFX-BioDn-5_st 947.2 14 0 20 20 18 0.7 3.1 Undef P
AFFX-BioDn-3_st 552.1 11 3 20 20 18 0.55 1.03 3.7 M
AFFX-CreX-5_st -7.9 6 8 20 20 18 0.3 -0.07 0.8 A
AFFX-CreX-3_st -310.3 6 7 20 20 18 0.3 -0.16 0.9 A
hum_alu_at 37.7 10 5 20 20 19 0.5 1.46 2 A
AFFX-DapX-5_at -43.1 9 4 20 20 19 0.45 1.18 2.3 A

Total number of rows: 7129

Table truncated, full table size 320 Kbytes.




Supplementary file Size Download File type/resource
GSM576.cel.gz 2.2 Mb (ftp)(http) CEL

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