|
| Status |
Public on May 30, 2012 |
| Title |
Aurka_R_-Dox_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AINV15
strain background: 129P2/OlaHsd
genotype/variation: Aurka
condition: -Dox
|
| Treatment protocol |
Each rescue ES clone was grown in +Dox (1ug/ml) media; Dox withdrawal was performed for 5days
|
| Growth protocol |
rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media supplemented with 15% FBS, 100 mM MEM non-essential amino acids, 0.1 mM 2-mercaptoethanol, 1 mM l-glutamine and 103 U ml-1 of LIF (Chemicon) and doxycycline (1ug/ml)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4C
|
| Data processing |
Data was VST transformed, RSN normalized in R using LUMI package
|
| |
|
| Submission date |
Aug 10, 2010 |
| Last update date |
May 30, 2012 |
| Contact name |
Yen-Sin Ang |
| Organization name |
Gladstone Institute, UCSF
|
| Street address |
1650 Owens Street
|
| City |
san francisco |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE23541 |
Regulation of Embryonic and Induced Pluripotency by Aurora Kinase-p53 Signaling |
|
