|
| Status |
Public on Aug 12, 2010 |
| Title |
liver_leucine_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
liver from leucine fed rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: liver
diet: leucine
age: 20 weeks
strain: Diet-induced obese Sprague-Dawley
gender: male
|
| Treatment protocol |
Rats were over-anaesthetized with isoflurane and killed via aortic cut. Liver was excised and submerged in liquid nitrogen immediately. Liver was stored at -80˚C until analysis.
|
| Growth protocol |
12 week old rats were provided one of 3 ad libitium diets for 8 weeks. Diets were prepared with 10% (by kcal) of either casein, leucine, or dairy protein.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver using Trizol Reagent (Invitrogen, Carlsbad, CA) and purified using the Qiagen RNeasy Miniprep (Qiagen, Mississauga, ON, Canada). RNA was quantified using Ribogreen and RT performed using the Omniscript RT Kit as per manufacturers directions (Qiagen, Mississauga, ON, Canada).
|
| Label |
biotin
|
| Label protocol |
As per Affymetrix protocol (Whole Transcript Sense Target Labeling Assay Manual for Rat Gene 1.0 ST Array available at www.affymetrix.com)
|
| |
|
| Hybridization protocol |
As per Affymetrix protocol (Whole Transcript Sense Target Labeling Assay Manual for Rat Gene 1.0 ST Array available at www.affymetrix.com)
|
| Scan protocol |
The chip scanned as per Affymetrix protocol using an Affymetrix Fluidics 450 scan station.
|
| Data processing |
The data was analyzed using Affymetrix Expression Console (algorithm-name: rma-gene-default, algorithm-version: 1.0, algorithm-param-quantification-name: med-polish).
|
| |
|
| Submission date |
Aug 11, 2010 |
| Last update date |
Aug 11, 2010 |
| Contact name |
Lindsay K Eller |
| E-mail(s) |
lkeller@ucalgary.ca
|
| Organization name |
University of Calgary
|
| Department |
Faculty of Kinesiology
|
| Lab |
Reimer Lab
|
| Street address |
2500 University Dr. NW
|
| City |
Calgary |
| State/province |
AB |
| ZIP/Postal code |
T2N1N4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE23559 |
The role of dietary leucine and dairy protein on hepatic gene expression |
|
