Comparison of gene expression in CD34+ cells from bone marrow and G-CSF mobilized peripheral blood by high density oligonucleotide array analysis. CD34 cells were isolated from granulocyte colony stimulating factor mobilized peripheral blood CD34 cells (GPBMC) by a combination of magnetic bead enrichment and flow cytometry. Total RNA from was isolated with Qiagen RNEasy columns, pooled with further samples and converted to biotin-labeled cRNA by standard Affymetrix protocols. For analysis the sample was scaled to an arbitrary value of 1000 by overall hybridization intensity to allow comparison of multiple samples. Chip hybridization parameters are: Background Avg: 652.56, Stdev: 13.33, Max: 674.1, Min: 635.3 Corner+ Avg:776, Count:32 Corner- Avg:44820, Count:32 BF= SDT=164.3(4.00Q) SRT=1.50 ABS={{3.0,4.0},{0.33,0.43},{0.90,1.30}} RawQ=23.77 QMult=2.80 RL=10.0 HZ=4 VZ=4 BG=2 CT=164.3 PCT=80 DD={{3.0,4.0},{0.33,0.43},{0.20,0.30},{0.90,1.30}} AvgNCT=200 FCMin=1.00 FCMax=1.00 STP=3.0 TGT=1000 NF=1.000000 SF=1.728414 SFGene=All