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Sample GSM580315 Query DataSets for GSM580315
Status Public on Dec 31, 2013
Title Treatment-8h-Rep 1
Sample type RNA
 
Source name Right cortex
Organism Rattus norvegicus
Characteristics strain: Wistar
gender: Male
weight: 250-300g
treatment: Treatment
treatment time-point: 8h
Treatment protocol Cerebral ischemia was induced by permanent occlusion of the left middle cerebral artery (MCA) using a subtemporal approach (Qu et al., 2006). Male Wistar rats (250 - 300 g) were anesthetized with ketamine (75mg/kg, intraperitoneal) and xylazine (10mg/kg, intraperitoneal). The MCA was exposed through a subtemporal craniectomy and cauterized from the point proximal to its origin to the point where it intersects the inferior cerebral vein. Sham group was operated in the same way as the experimental group without the occlusion of MCA.
Growth protocol All procedures performed in this study were approved by the Institutional Animal Care and Use Committee of the National University of Singapore.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the conventional Trizol method and cleaned up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol with a hybridization duration of 17h.
Scan protocol Standard Illumina scanning protocol with a pmt value=503 and scan factor=0.74.
Description Replicate 1
5038770021_E
Data processing The data were normalised using median normalisation with GeneSpring Ver 7.3
 
Submission date Aug 16, 2010
Last update date Dec 31, 2013
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
 
Platform ID GPL6101
Series (2)
GSE23651 Global transcriptomic profiling of permanent focal ischemia in an in vivo rat model
GSE25676 Global gene regulation in permanent focal ischemia in an in vivo rat model

Data table header descriptions
ID_REF
VALUE Median normalisation, p<0.05

Data table
ID_REF VALUE
ILMN_1363499 195.5979
ILMN_1363498 68.56208
ILMN_1363497 65.21958
ILMN_1363496 147.1242
ILMN_1363495 72.628784
ILMN_1363493 64.009834
ILMN_1363492 94.95559
ILMN_1363491 116.013084
ILMN_1363490 1718.4777
ILMN_1375469 315.20895
ILMN_1363489 63.1425
ILMN_1363488 2123.0518
ILMN_1363486 269.0426
ILMN_1363485 64.34195
ILMN_1363482 58.561546
ILMN_1363481 1324.6813
ILMN_1363480 454.29254
ILMN_1349199 175.79012
ILMN_1349198 9865.794
ILMN_1349197 1118.736

Total number of rows: 22523

Table truncated, full table size 494 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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