|
| Status |
Public on Jul 02, 2013 |
| Title |
MEF b1wt_L0 rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse embryonic fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: B1 wildtype
cell type: Mouse embryonic fibroblast
developmental stage: prepared from E13.11
sex: female
immortalization: SV-40 large T
|
| Treatment protocol |
The b1YYFF integrin mutation renders MEFs incapable of executing cytokinesis on b1 integrin specific matrixes. Repeated cytokinesis failure was induced by plating the clones on the β1 specific matrix component laminin in serum-free hybridoma medium over night. Normal medium was added the following day to allow for adhesion via β1 and β3 integrins and thus execution of normal cytokinesis. This procedure was repeated 4 or 8 times after which the cells were grown under normal conditions on plastic.
|
| Growth protocol |
MEFs were grown in high clucose DMEM supplemented with 10% FBS, 1% L-glutamine and 1% Penicillin/Streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAGEN RNeasy mini handbook protocol.
|
| Label |
biotin
|
| Label protocol |
For the Illumina gene expression analysis, 100 ng of purified total RNA was amplified and biotinylated in an overnight in vitro transcription reaction using the Illumina RNA TotalPrepTM amplification kit (Ambion, Applied Biosystems).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
16A3Lo_1
|
| Data processing |
The data were normalized using quantile normalisation with limma in R
|
| |
|
| Submission date |
Aug 20, 2010 |
| Last update date |
Jul 02, 2013 |
| Contact name |
Gunilla Högnäs |
| E-mail(s) |
Ext-Gunilla.Hognas@vtt.fi
|
| Organization name |
VTT
|
| Department |
Medical Biotechnology
|
| Street address |
Tykistökatu 6 B
|
| City |
Turku |
| ZIP/Postal code |
20521 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE23729 |
The effect of aneuploidy on gene expression |
|
