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Sample GSM585577 Query DataSets for GSM585577
Status Public on Jul 02, 2013
Title MEF b1wt_L0 rep 2
Sample type RNA
Source name Mouse embryonic fibroblast
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: B1 wildtype
cell type: Mouse embryonic fibroblast
developmental stage: prepared from E13.12
sex: female
immortalization: SV-40 large T
Treatment protocol The b1YYFF integrin mutation renders MEFs incapable of executing cytokinesis on b1 integrin specific matrixes. Repeated cytokinesis failure was induced by plating the clones on the β1 specific matrix component laminin in serum-free hybridoma medium over night. Normal medium was added the following day to allow for adhesion via β1 and β3 integrins and thus execution of normal cytokinesis. This procedure was repeated 4 or 8 times after which the cells were grown under normal conditions on plastic.
Growth protocol MEFs were grown in high clucose DMEM supplemented with 10% FBS, 1% L-glutamine and 1% Penicillin/Streptomycin
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using QIAGEN RNeasy mini handbook protocol.
Label biotin
Label protocol For the Illumina gene expression analysis, 100 ng of purified total RNA was amplified and biotinylated in an overnight in vitro transcription reaction using the Illumina RNA TotalPrepTM amplification kit (Ambion, Applied Biosystems).
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 16A3Lo_2
Data processing The data were normalized using quantile normalisation with limma in R
Submission date Aug 20, 2010
Last update date Jul 02, 2013
Contact name Gunilla Högnäs
Organization name VTT
Department Medical Biotechnology
Street address Tykistökatu 6 B
City Turku
ZIP/Postal code 20521
Country Finland
Platform ID GPL6885
Series (1)
GSE23729 The effect of aneuploidy on gene expression

Data table header descriptions
VALUE quantile normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 2079.868 0
ILMN_2721178 547.5482 0
ILMN_3033922 873.9055 0
ILMN_3092673 2803.036 0
ILMN_2816356 196.5204 0
ILMN_2808939 876.2684 0
ILMN_2634564 1062.372 0
ILMN_2737647 72.00967 0.3132832
ILMN_2734484 246.6962 0
ILMN_2952292 247.3293 0
ILMN_2699078 62.41084 0.5426065
ILMN_1213681 292.688 0
ILMN_2735413 68.71214 0.3796993
ILMN_2735415 68.62417 0.3809524
ILMN_2891688 596.9949 0
ILMN_2637698 1355.488 0
ILMN_2674228 530.7706 0
ILMN_2601546 106.6997 0.04260652
ILMN_1230831 83.35365 0.1466165
ILMN_2848071 82.75142 0.1516291

Total number of rows: 25697

Table truncated, full table size 737 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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