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Sample GSM586063 Query DataSets for GSM586063
Status Public on Aug 27, 2011
Title soy protein+isoflavone diet, biological rep4
Sample type RNA
 
Source name rat liver, soy protein+isoflavone diet fed for 14 days
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
sex: male
tissue: liver
Treatment protocol Each group was fed a diet containing 200 g/kg of protein in the form of casein, soy protein, or freeze-dried tofu. The experimental diets were as follows: 1) casein diet (C); 2) C containing isoflavone (CI); 3) soy protein diet (S); 4) S containing isoflavone (SI); 5) a diet containing 100 g/kg each of protein derived from casein and freeze-dried tofu (T10); and 6) a diet containing 200 g/kg of protein derived from freeze-dried tofu (T20) (Table 1). CI and SI were supplemented with a mixture of isoflavones to match the isoflavone level of T20.
Growth protocol Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ÂșC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
Extracted molecule total RNA
Extraction protocol At the end of the experimental period, the animals were sacrificed by bleeding from the abdominal aorta under diethyl ether anesthesia, and the liver was quickly excised. Hepatic total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Ten micrograms of total RNA was processed to prepare biotinylated cRNA using One-Cycle Target Labeling and Control Reagents (Afflymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C, according to the manual supplied by Affymetrix (P/N 702232 Rev. 2). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned with a GCS 3000 scanner (GCOS V1.2, Affymetrix).
Description The following parameters for data distribution were found by using SuperNORM data service.
under limit: -0.677428187182076
upper limit: 2.54902877609805
saturation: 2.99247546773371
gamma: 50.7010461092227
sigma: 0.784372127799414
mu: 1.9030062614181
Data processing The data were analyzed by using SuperNORM (V 1.7, Skylight Biotech Inc.) accoring to a parametric method (Konishi, T., 2008, Stat Appl Genet Mol Biol 7: Article 25.). A row of the data table presents z-scores of the PM data of a gene, in accordance with the order of the Cell appears in the CDF file. The PM data are further summarized to find z-scores and pseudo data value of a gene. The signal intensity represents the pseudo data, of which center of intensity for each array was arbitrarily set to 256. For details of the methodology, visit http://www.super-norm.com/eng/index.html.
 
Submission date Aug 23, 2010
Last update date Aug 27, 2011
Contact name Tomokazu Konishi
E-mail(s) konishi@akita-pu.ac.jp
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
 
Platform ID GPL1355
Series (1)
GSE23748 Tofu decreases serum lipid levels and modulates hepatic gene expression involved in lipid metabolism in rats

Data table header descriptions
ID_REF
VALUE Normalized PM 'Pseudodata' obtained in the pivot output of SuperNORM. (z-scores were also supplied with the pseudo data)
detection call
z-score

Data table
ID_REF VALUE detection call z-score
1367452_at 6012.1 P 1.706
1367453_at 3820.7 P 1.461
1367454_at 3232.7 P 1.37
1367455_at 6251.3 P 1.727
1367456_at 12022.7 P 2.08
1367457_at 1700.3 P 1.023
1367458_at 1938.6 P 1.094
1367459_at 19370.3 P 2.338
1367460_at 4394 P 1.536
1367461_at 1723.5 P 1.031
1367462_at 6079.6 P 1.712
1367463_at 7700.8 P 1.84
1367464_at 3452.2 P 1.406
1367465_at 5788.2 P 1.685
1367466_at 3902.2 P 1.472
1367467_at 9183.6 P 1.935
1367468_at 3131 P 1.353
1367469_at 14336.8 P 2.175
1367470_at 3454.2 P 1.406
1367471_at 2853 P 1.303

Total number of rows: 31099

Table truncated, full table size 798 Kbytes.




Supplementary file Size Download File type/resource
GSM586063.CEL.gz 2.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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