NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM586074 Query DataSets for GSM586074
Status Public on Aug 27, 2011
Title tofu diet, biological rep5
Sample type RNA
 
Source name rat liver, tofu diet fed for 14 days
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
sex: male
tissue: liver
Treatment protocol Each group was fed a diet containing 200 g/kg of protein in the form of casein, soy protein, or freeze-dried tofu. The experimental diets were as follows: 1) casein diet (C); 2) C containing isoflavone (CI); 3) soy protein diet (S); 4) S containing isoflavone (SI); 5) a diet containing 100 g/kg each of protein derived from casein and freeze-dried tofu (T10); and 6) a diet containing 200 g/kg of protein derived from freeze-dried tofu (T20) (Table 1). CI and SI were supplemented with a mixture of isoflavones to match the isoflavone level of T20.
Growth protocol Male Sprague-Dawley rats were obtained from Charles River Japan (Kanagawa, Japan) at 4 week of age. Animals were housed individually in a room with a controlled temperature (20-22ÂșC), humidity (55-65%), and 12-h-light/-dark cycle. During their adaptation to the conditions, rats were fed a commercial non-purified diet (Type NMF; Oriental Yeast Co., Tokyo, Japan) for 5 days. Rats were allowed free access to diet and water.
Extracted molecule total RNA
Extraction protocol At the end of the experimental period, the animals were sacrificed by bleeding from the abdominal aorta under diethyl ether anesthesia, and the liver was quickly excised. Hepatic total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Ten micrograms of total RNA was processed to prepare biotinylated cRNA using One-Cycle Target Labeling and Control Reagents (Afflymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C, according to the manual supplied by Affymetrix (P/N 702232 Rev. 2). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned with a GCS 3000 scanner (GCOS V1.2, Affymetrix).
Description The following parameters for data distribution were found by using SuperNORM data service.
under limit: -0.402185413063392
upper limit: 2.62548510224213
saturation: 2.99247546773371
gamma: 52.4558035154239
sigma: 0.796201600637588
mu: 1.89024841313523
Data processing The data were analyzed by using SuperNORM (V 1.7, Skylight Biotech Inc.) accoring to a parametric method (Konishi, T., 2008, Stat Appl Genet Mol Biol 7: Article 25.). A row of the data table presents z-scores of the PM data of a gene, in accordance with the order of the Cell appears in the CDF file. The PM data are further summarized to find z-scores and pseudo data value of a gene. The signal intensity represents the pseudo data, of which center of intensity for each array was arbitrarily set to 256. For details of the methodology, visit http://www.super-norm.com/eng/index.html.
 
Submission date Aug 23, 2010
Last update date Aug 27, 2011
Contact name Tomokazu Konishi
E-mail(s) konishi@akita-pu.ac.jp
Phone +81-18-872-1603
Organization name Akita Prefectural University
Department Bioresource Sciences
Lab Molecular Genetics
Street address Shimoshinjyo Nishi
City Akita
State/province Akita
ZIP/Postal code 010-0195
Country Japan
 
Platform ID GPL1355
Series (1)
GSE23748 Tofu decreases serum lipid levels and modulates hepatic gene expression involved in lipid metabolism in rats

Data table header descriptions
ID_REF
VALUE Normalized PM 'Pseudodata' obtained in the pivot output of SuperNORM. (z-scores were also supplied with the pseudo data)
detection call
z-score

Data table
ID_REF VALUE detection call z-score
1367452_at 5387.8 P 1.647
1367453_at 3796 P 1.457
1367454_at 2727.9 P 1.279
1367455_at 5846.7 P 1.691
1367456_at 11376.7 P 2.05
1367457_at 1694.6 P 1.021
1367458_at 2072.2 P 1.13
1367459_at 17973 P 2.298
1367460_at 3750.5 P 1.451
1367461_at 1584.3 P 0.9851
1367462_at 6023.2 P 1.707
1367463_at 7926.5 P 1.855
1367464_at 3292.5 P 1.38
1367465_at 4919.9 P 1.597
1367466_at 3691.6 P 1.442
1367467_at 9203.1 P 1.936
1367468_at 3197 P 1.364
1367469_at 9883 P 1.974
1367470_at 3872 P 1.468
1367471_at 2750.8 P 1.283

Total number of rows: 31099

Table truncated, full table size 798 Kbytes.




Supplementary file Size Download File type/resource
GSM586074.CEL.gz 2.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap