|
| Status |
Public on Sep 03, 2010 |
| Title |
ventral SKPs sample 3 |
| Sample type |
RNA |
| |
|
| Source name |
ventral SKPs sample 3
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell passage: twiced-passaged cells
strain: Sprague Dawley
cell type: ventral SKPs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol (Invitrogen), as per the manufacturer’s instructions, followed by the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Rat Gene 1.0 ST Arrays (Affymetrix) and scanned at the HSC Microarray Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software; gene-core analysis
|
| Data processing |
The data were background corrected and normalized using standard RMA procedure implemented in the Affymetrix Expression Console software. The preprocessed data were analyzed using the LIMMA Bioconductor package to identify genes that show significant evidence of differential expression between SKPs and MSCs.
|
| |
|
| Submission date |
Sep 02, 2010 |
| Last update date |
Sep 02, 2010 |
| Contact name |
Freda Miller |
| E-mail(s) |
fredam@sickkids.ca
|
| Organization name |
Hospital for Sick Children
|
| Street address |
555 University Avenue
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE23954 |
CONVERGENT GENESIS OF AN ADULT NEURAL CREST-LIKE DERMAL STEM CELL FROM DISTINCT DEVELOPMENTAL ORIGINS |
|
