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Sample GSM593159 Query DataSets for GSM593159
Status Public on Sep 14, 2010
Title Sqadipose_t4_Con_a3
Sample type RNA
 
Source name Subcutaneous adipose tissue at 12 weeks
Organism Canis lupus familiaris
Characteristics tissue: subcutaneous adipose
animal id: 3
time point: 12 weeks
treatment: Control
batch: 4
breed: Beagle
Treatment protocol Dogs were randomized to either ad libitum (n=5) or body weight maintenance (n=4) feeding. All dogs were fed Purina® Pro Plan® Performance Formula. All subcutaneous adipose samples were collected after an overnight fasting period.
Growth protocol Dogs were fed ad libitum (n=5) or to maintain baseline body weight (control; n=4).
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol as suggested by the manufacturer.
Label biotin
Label protocol Biotin-labeled cRNA samples were made using Affymetrix GeneChip Expression 3'-Amplification Reagents (One-Cycle Target Labeling and Control Reagents package) according to the manufacturer's instructions.
 
Hybridization protocol Purified, fragmented cRNA was hybridized to Affymetrix's Canine 2.0 Genome Array using Affymetrix's GeneChip Fluidics Station 450, as per Affymetrix's instructions.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
Description Control dog gene expression data from subcutaneous adipose tissure at 12 weeks
Data processing Pre-processing was done using the GCRMA algorithm in R and Bioconductor packages.
 
Submission date Sep 13, 2010
Last update date Sep 13, 2010
Contact name Kelly Scott Swanson
E-mail(s) ksswanso@uiuc.edu
Organization name University of Illinois
Street address 1207 W. Gregory Dr.
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platform ID GPL3738
Series (1)
GSE24099 Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression

Data table header descriptions
ID_REF
VALUE GCRMA log2 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 9.036427526
AFFX-BioB-5_at 9.053108507
AFFX-BioB-M_at 10.13196553
AFFX-BioC-3_at 11.34978096
AFFX-BioC-5_at 10.41514748
AFFX-BioDn-3_at 12.76773799
AFFX-BioDn-5_at 12.44708989
AFFX-Cf_Actin_3_at 14.37348338
AFFX-Cf_Actin_5_s_at 14.35450587
AFFX-Cf_Actin_M_at 12.19546653
AFFX-Cf_AdrenRecpt_3_at 3.136288223
AFFX-Cf_AdrenRecpt_5_at 1.867464476
AFFX-Cf_AdrenRecpt_M_at 3.676005539
AFFX-Cf_G-6-PDH_3_at 10.30538725
AFFX-Cf_G-6-PDH_5_at 7.805420534
AFFX-Cf_G-6-PDH_M_at 7.295821677
AFFX-Cf_Gapdh_3_at 15.09931154
AFFX-Cf_Gapdh_5_at 14.84098304
AFFX-Cf_Gapdh_M_at 13.34490678
AFFX-Cf_P450-2E1_3_s_at 2.522265803

Total number of rows: 43035

Table truncated, full table size 1356 Kbytes.




Supplementary file Size Download File type/resource
GSM593159_2481_E_30_Canine_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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