|
| Status |
Public on Sep 14, 2010 |
| Title |
Sqadipose_t4_Adlib_a2 |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous adipose tissue at 12 weeks
|
| Organism |
Canis lupus familiaris |
| Characteristics |
tissue: subcutaneous adipose
animal id: 2
time point: 12 weeks
treatment: Ad libitum
batch: 5
breed: Beagle
|
| Treatment protocol |
Dogs were randomized to either ad libitum (n=5) or body weight maintenance (n=4) feeding. All dogs were fed Purina® Pro Plan® Performance Formula. All subcutaneous adipose samples were collected after an overnight fasting period.
|
| Growth protocol |
Dogs were fed ad libitum (n=5) or to maintain baseline body weight (control; n=4).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol as suggested by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA samples were made using Affymetrix GeneChip Expression 3'-Amplification Reagents (One-Cycle Target Labeling and Control Reagents package) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Purified, fragmented cRNA was hybridized to Affymetrix's Canine 2.0 Genome Array using Affymetrix's GeneChip Fluidics Station 450, as per Affymetrix's instructions.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner 3000
|
| Description |
Ad libitum fed dog gene expression data from subcutaneous adipose tissure at 12 weeks
|
| Data processing |
Pre-processing was done using the GCRMA algorithm in R and Bioconductor packages.
|
| |
|
| Submission date |
Sep 13, 2010 |
| Last update date |
Sep 13, 2010 |
| Contact name |
Kelly Scott Swanson |
| E-mail(s) |
ksswanso@uiuc.edu
|
| Organization name |
University of Illinois
|
| Street address |
1207 W. Gregory Dr.
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE24099 |
Effects of ad libitum feeding on canine subcutaneous adipose tissue gene expression |
|
