|
| Status |
Public on May 01, 2011 |
| Title |
heart_TAC4w_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse wildtype ventricle
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: heart ventricle
gender: male
stress: transverse aortic constriction
time: 4 week
|
| Treatment protocol |
The wile-type mice were subject to sham- or TAC-operation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA as described by Affymetrix.
|
| |
|
| Hybridization protocol |
RNA samples were hybridizaed with Affymetrix expression arrays offering whole-transcript coverage. After hybridization, non-specific binding was washed off according to the manufacturer's protocol.
|
| Scan protocol |
Affymetrix arrays were scanned with the Affymetrix GeneArray scanner.
|
| Description |
4week pressure overload
BTIA_EXON_4WTAC2_053008.CEL
|
| Data processing |
In the Expression Console software, hybridization intensity data were normalized using the Robust Multichip Average (RMA) method.
|
| |
|
| Submission date |
Sep 21, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Jiyeon Park |
| E-mail(s) |
parkji7@snu.ac.kr
|
| Organization name |
Seoul National Univerisy
|
| Department |
Biomedical Informatics
|
| Lab |
Bioinformatics
|
| Street address |
103 Daehak-ro, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE24242 |
Gene and exon expression change in cardiac hypertrophy with pressure overload |
|
