|
| Status |
Public on Sep 23, 2010 |
| Title |
mouse E12.5 ureteric bud stalk_biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
microdissected ureteric bud stalks
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E12.5
cell type: microdissected ureteric bud stalks
|
| Growth protocol |
A subclone of mIMCD-3 cells was grown to confluency in Dulbecco's Modified Eagle Medium (DMEM). The detailed protocol for microdissection of E12.5 embryonic kidneys was described previously (Schmidt-Ott, K. M., Yang, J., Chen, X., Wang, H., Paragas, N., Mori, K., Li, J. Y., Lu, B., Costantini, F., Schiffer, M. et al. (2005). Novel regulators of kidney development from the tips of the ureteric bud. J Am Soc Nephrol 16, 1993-2002)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Fifteen micrograms of fragmented target cRNA per chip was used in duplicate hybridizations according to standard Affymetrix protocols (Affymetrix Genechip Fluidics Workstation).
|
| Scan protocol |
Hybridized chips were read on an Affymetrix Scanner Type M10.
|
| Description |
Gene expression data from mouse E12.5 microdissected ureteric bud stalk
|
| Data processing |
Robust multichip analysis (RMA) was carried out using the Affymetrix microarray *.cel files. The RMA data were generated using RMA Express.
|
| |
|
| Submission date |
Sep 23, 2010 |
| Last update date |
Sep 23, 2010 |
| Contact name |
Kai Martin Schmidt-Ott |
| Organization name |
Max-Delbrueck Center for Molecular Medicine
|
| Department |
Differentiation and Regeneration of Kidney Epithelia
|
| Lab |
Kai Schmidt-Ott
|
| Street address |
Robert-Roessle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE24295 |
Gene expression in epithelial and non-epithelial cells of renal origin |
|
| Relations |
| Reanalysis of |
GSM35260 |
