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Sample GSM602030 Query DataSets for GSM602030
Status Public on Oct 01, 2011
Title IPO-24
Sample type RNA
 
Source name Liver tissue
Organism Rattus norvegicus
Characteristics strain: Wistar
gender: male
ischemia protocol: 30 min of total liver ischemia + IPO
Treatment protocol Animals were anaesthetized using 0.75 ml/kg Hypnorm s.c. (fentanyl/fluanisone; Jansen Pharma, Birkeroed, Denmark) and 4 mg/kg Midazolam s.c. (dormicum; La Roche, Basel, Switzerland) and placed on a heated pad. A midline laparotomy was performed and the hepatoduodenal ligament was mobilised. Liver biopsies were sampled from the right lobe and immediately snap frozen in liquid nitrogen, and stored at -80°C until analysis.
Growth protocol Male Wistar rats, weighing 300–350 g, (M&B Taconic, Eiby, Denmark) were housed in standard animal laboratories with the temperature maintained at 23°C and artificial 12-hour light-dark cycles, and with free access to food and water.
Extracted molecule total RNA
Extraction protocol Biopsies were homogenized in RLT plus buffer on Qiagen Tissuelyser. Total RNA was extracted on the QIAsymphony SP using the QIAsymphony RNA extraction kit.
Label Biotin
Label protocol 150 ng of total RNA was used as target preparation in the starting material for the cDNA Syntesis Kit and WT Terminal Labeling Kit (affymetrix).
 
Hybridization protocol The target was loaded onto the GeneChip and hybridised for 16 h. The arrays were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using Affymetrix 7G GeneChip Scanner.
Description IPO-24
Data processing Raw image files from the quantitative scannings were analysed by Affymetrix Gene Expression Analysis Software and data were normalized using the RMA in Affymetrix Expression Console.
 
Submission date Sep 29, 2010
Last update date Oct 01, 2011
Contact name Anders Riegels Knudsen
E-mail(s) auknudsen@gmail.com
Organization name Aarhus University Hospital
Department Department of Surgery, Section for upper GI surgery
Street address Nørrebrogade 44, building 1c, LVN-research
City Aarhus C
ZIP/Postal code 8000
Country Denmark
 
Platform ID GPL6247
Series (1)
GSE24430 Ischemic Pre- and Post Conditioning has pronounced effects on Gene Expression Profiles in the Rat Liver after Ischemia/Reperfusion

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10701620 3.235937
10701630 2.599599
10701632 3.001249
10701636 2.956413
10701643 3.143426
10701648 3.012166
10701654 2.83591
10701663 5.08575
10701666 2.481351
10701668 3.530521
10701671 3.093299
10701674 3.201028
10701679 2.690341
10701684 4.918652
10701689 3.251572
10701691 4.22842
10701697 5.626909
10701699 6.952907
10701709 6.724486
10701714 4.844776

Total number of rows: 29215

Table truncated, full table size 510 Kbytes.




Supplementary file Size Download File type/resource
GSM602030.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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