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Sample GSM602053 Query DataSets for GSM602053
Status Public on Oct 01, 2011
Title Sham-47
Sample type RNA
 
Source name Liver tissue
Organism Rattus norvegicus
Characteristics strain: Wistar
gender: male
ischemia protocol: only laparotomy
Treatment protocol Animals were anaesthetized using 0.75 ml/kg Hypnorm s.c. (fentanyl/fluanisone; Jansen Pharma, Birkeroed, Denmark) and 4 mg/kg Midazolam s.c. (dormicum; La Roche, Basel, Switzerland) and placed on a heated pad. A midline laparotomy was performed and the hepatoduodenal ligament was mobilised. Liver biopsies were sampled from the right lobe and immediately snap frozen in liquid nitrogen, and stored at -80°C until analysis.
Growth protocol Male Wistar rats, weighing 300–350 g, (M&B Taconic, Eiby, Denmark) were housed in standard animal laboratories with the temperature maintained at 23°C and artificial 12-hour light-dark cycles, and with free access to food and water.
Extracted molecule total RNA
Extraction protocol Biopsies were homogenized in RLT plus buffer on Qiagen Tissuelyser. Total RNA was extracted on the QIAsymphony SP using the QIAsymphony RNA extraction kit.
Label Biotin
Label protocol 150 ng of total RNA was used as target preparation in the starting material for the cDNA Syntesis Kit and WT Terminal Labeling Kit (affymetrix).
 
Hybridization protocol The target was loaded onto the GeneChip and hybridised for 16 h. The arrays were washed and stained in the Affymetrix Fluidics Station.
Scan protocol GeneChips were scanned using Affymetrix 7G GeneChip Scanner.
Description Sham-47
Data processing Raw image files from the quantitative scannings were analysed by Affymetrix Gene Expression Analysis Software and data were normalized using the RMA in Affymetrix Expression Console.
 
Submission date Sep 29, 2010
Last update date Oct 01, 2011
Contact name Anders Riegels Knudsen
E-mail(s) auknudsen@gmail.com
Organization name Aarhus University Hospital
Department Department of Surgery, Section for upper GI surgery
Street address Nørrebrogade 44, building 1c, LVN-research
City Aarhus C
ZIP/Postal code 8000
Country Denmark
 
Platform ID GPL6247
Series (1)
GSE24430 Ischemic Pre- and Post Conditioning has pronounced effects on Gene Expression Profiles in the Rat Liver after Ischemia/Reperfusion

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10701620 3.431146
10701630 2.701318
10701632 2.92771
10701636 3.141937
10701643 3.232178
10701648 2.942083
10701654 2.975892
10701663 4.694304
10701666 2.819063
10701668 3.504645
10701671 3.283958
10701674 3.330452
10701679 2.751448
10701684 5.331508
10701689 4.81184
10701691 4.709756
10701697 5.460123
10701699 6.037033
10701709 6.950236
10701714 4.749822

Total number of rows: 29215

Table truncated, full table size 510 Kbytes.




Supplementary file Size Download File type/resource
GSM602053.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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