|
| Status |
Public on Dec 22, 2011 |
| Title |
doubleKO Dex - A |
| Sample type |
RNA |
| |
|
| Source name |
doubleKO Dex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
genotype/variation: Cry1-/-Cry2-/-
dev stage: E15.5 embryos
tissue: primary MEFs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Cry1/2 double KO MEFs treated with Dex, biological replicate 1
|
| Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using the VAMPIRE Bayesian model with a 2-sided Bonferroni-corrected threshold of 0.05.
|
| |
|
| Submission date |
Sep 30, 2010 |
| Last update date |
Dec 22, 2011 |
| Contact name |
Ruth T Yu |
| Organization name |
Salk Institute
|
| Department |
Gene Expression Lab
|
| Lab |
Ronald Evans
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE24469 |
Cryptochromes mediate rhythmic repression of the glucocorticoid receptor |
|
