|
| Status |
Public on Dec 18, 2011 |
| Title |
Wild type mammary endothelial cells, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
wild type mammary endothelial cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVBN
age: 9 weeks
tissue: Mammary gland
genotype/variation: PtenloxP/loxP
|
| Treatment protocol |
Fibroblasts and epithelial cells were grown in 10% FBS DMEM in a 37° incubator with 7% CO2
|
| Growth protocol |
Fibroblasts and epithelial cells were cultured using a modified protocol for gravity separation. Endothelial cells were directly isolated from tissue using FACS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2005, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15ug of cRNA was hybridized for 16 hours at 45°C on Affymetrix Mouse Exon 1.0 ST Array GeneChips. GeneCHips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetric GeneChip Scanner 3000
|
| Description |
Gene expression data from wild type mammary endothelial cells
|
| Data processing |
Data were processed using Affymetrix Expression Console software. Robust Multichip Average (RMA) method was used to do background correction, quantile normalization, and expression level summarization.
|
| |
|
| Submission date |
Oct 04, 2010 |
| Last update date |
Dec 18, 2011 |
| Contact name |
Julie Wallace |
| E-mail(s) |
Julie.Reeser@osumc.edu
|
| Organization name |
OSUCCC
|
| Street address |
460 W. 12th Ave 840 BRT
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE24501 |
Pten signaling in fibroblasts effects gene expression in endothelial and epithelial cells |
|
