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Sample GSM60417 Query DataSets for GSM60417
Status Public on Dec 06, 2005
Title tumor immune evasion - P3 cell line
Sample type RNA
Source name mouse cancer cell line, P3
Organism Mus musculus
Characteristics strain - C57BL/6
gender - female
tissue - P0/TC-1 is a cancer cell line derived from lung fibroblasts. P3 is derived from P0.
Treatment protocol P3 is derived from P0 through 3 rounds of in vivo immune selection. P3 is a highly immune resistant cell line.
Growth protocol The cell line was cultured in vitro in 10-cm dishes until 70-75% confluency and then total RNA was isolated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen), followed by a clean-up step using RNeasy mini kit (Qiagen).
Label biotin-labeled cRNA, detected with streptavidin phycoerythin conjugate
Description This is one of 4 biological replicates for P3 cell line.
Data processing background correction made with RMA, Quantile Normalization, GC-RMA
Submission date Jun 06, 2005
Last update date Jun 07, 2005
Contact name Ken-Yu Lin
Phone 410-614-4906
Organization name Johns Hopkins University School of Medicine
Department Pathology
Lab T.-C. Wu
Street address 720 Rutland Ave, Ross 512
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL1261
Series (1)
GSE2774 Identification of tumor immune evasion mechanism using P0 and P3 cell lines

Data table header descriptions
VALUE data with quantile normalization

Data table
1415670_at 2769.098516
1415671_at 646.638141
1415672_at 2894.12359
1415673_at 679.178079
1415674_a_at 633.739758
1415675_at 568.721427
1415676_a_at 7171.455253
1415677_at 901.112564
1415678_at 1217.277986
1415679_at 1661.637497
1415680_at 2887.926183
1415681_at 712.07152
1415682_at 1116.028651
1415683_at 2221.12256
1415684_at 320.174343
1415685_at 305.073325
1415686_at 1347.161879
1415687_a_at 3556.561236
1415688_at 1056.014522
1415689_s_at 105.079408

Total number of rows: 45101

Table truncated, full table size 932 Kbytes.

Supplementary data files not provided

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