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Sample GSM60418 Query DataSets for GSM60418
Status Public on Dec 06, 2005
Title tumor immune evasion - P3 cell line-replicate 2
Sample type RNA
Source name mouse cancer cell line, P3
Organism Mus musculus
Characteristics strain - C57BL/6
gender - female
tissue - P0/TC-1 is a cancer cell line derived from lung fibroblasts. P3 is derived from P0.
Treatment protocol P3 is derived from P0 through 3 rounds of in vivo immune selection. P3 is a highly immune resistant cell line.
Growth protocol The cell line was cultured in vitro in 10-cm dishes until 70-75% confluency and then total RNA was isolated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen), followed by a clean-up step using RNeasy mini kit (Qiagen).
Label biotin-labeled cRNA, detected with streptavidin phycoerythin conjugate
Description This is one of 4 biological replicates for P3 cell line.
Data processing background correction made with RMA, Quantile Normalization, GC-RMA
Submission date Jun 06, 2005
Last update date Jun 07, 2005
Contact name Ken-Yu Lin
Phone 410-614-4906
Organization name Johns Hopkins University School of Medicine
Department Pathology
Lab T.-C. Wu
Street address 720 Rutland Ave, Ross 512
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL1261
Series (1)
GSE2774 Identification of tumor immune evasion mechanism using P0 and P3 cell lines

Data table header descriptions
VALUE data with quantile normalization

Data table
1415670_at 2832.916431
1415671_at 613.937247
1415672_at 3131.714161
1415673_at 811.977101
1415674_a_at 618.206484
1415675_at 537.031842
1415676_a_at 7042.547843
1415677_at 970.261018
1415678_at 1164.098026
1415679_at 1576.007565
1415680_at 2711.385555
1415681_at 680.295626
1415682_at 1020.343669
1415683_at 2093.184776
1415684_at 341.583334
1415685_at 281.969376
1415686_at 1370.310569
1415687_a_at 3785.424956
1415688_at 988.497123
1415689_s_at 103.652888

Total number of rows: 45101

Table truncated, full table size 932 Kbytes.

Supplementary data files not provided

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