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Sample GSM60420 Query DataSets for GSM60420
Status Public on Dec 06, 2005
Title tumor immune evasion - P3 cell line-replicate 3
Sample type RNA
Source name mouse cancer cell line, P3
Organism Mus musculus
Characteristics strain - C57BL/6
gender - female
tissue - P0/TC-1 is a cancer cell line derived from lung fibroblasts. P3 is derived from P0.
Treatment protocol P3 is derived from P0 through 3 rounds of in vivo immune selection. P3 is a highly immune resistant cell line.
Growth protocol The cell line was cultured in vitro in 10-cm dishes until 70-75% confluency and then total RNA was isolated.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIzol reagent (Invitrogen), followed by a clean-up step using RNeasy mini kit (Qiagen).
Label biotin-labeled cRNA, detected with streptavidin phycoerythin conjugate
Description This is one of 4 biological replicates for P3 cell line.
Data processing background correction made with RMA, Quantile Normalization, GC-RMA
Submission date Jun 06, 2005
Last update date Jun 07, 2005
Contact name Ken-Yu Lin
Phone 410-614-4906
Organization name Johns Hopkins University School of Medicine
Department Pathology
Lab T.-C. Wu
Street address 720 Rutland Ave, Ross 512
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL1261
Series (1)
GSE2774 Identification of tumor immune evasion mechanism using P0 and P3 cell lines

Data table header descriptions
VALUE data with quantile normalization

Data table
1415670_at 2839.754723
1415671_at 627.081278
1415672_at 2941.135791
1415673_at 683.616667
1415674_a_at 579.113231
1415675_at 524.01914
1415676_a_at 7215.163041
1415677_at 949.493207
1415678_at 1179.016758
1415679_at 1612.718075
1415680_at 2696.087056
1415681_at 646.243772
1415682_at 1003.695596
1415683_at 2181.549761
1415684_at 351.697852
1415685_at 296.609998
1415686_at 1377.806798
1415687_a_at 3948.064997
1415688_at 976.064743
1415689_s_at 97.126637

Total number of rows: 45101

Table truncated, full table size 932 Kbytes.

Supplementary data files not provided

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