|
| Status |
Public on Jun 06, 2006 |
| Title |
WT (w1118) control replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
embryos, WT (w1118), control
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Wild type (w1118), embryos 4-6.5 hours after egg laying, control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 1 gram of wild-type (w1118 strain) embryos was collected 0-2.5 hours after egg laying. The embryos were aged for 2.5 hours at 25C, mock-treated, and allowed a 1.5 hour recovery prior to dechorionation. Total RNA was isolated using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Wild type (w1118) embryos
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Jun 08, 2005 |
| Last update date |
Jun 08, 2005 |
| Contact name |
John M Abrams |
| E-mail(s) |
John.Abrams@UTSouthwestern.edu
|
| Phone |
214-648-9226
|
| Fax |
214-648-8694
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Cell Biology
|
| Lab |
Abrams
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9039 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE2780 |
Radiation-induced Dmp53-dependent expression profile |
|
