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Sample GSM60480 Query DataSets for GSM60480
Status Public on Jun 06, 2006
Title WT (w1118) control replicate 2
Sample type RNA
 
Source name embryos, WT (w1118), control
Organism Drosophila melanogaster
Characteristics Wild type (w1118), embryos 4-6.5 hours after egg laying, control
Extracted molecule total RNA
Extraction protocol Approximately 1 gram of wild-type (w1118 strain) embryos was collected 0-2.5 hours after egg laying. The embryos were aged for 2.5 hours at 25C, mock-treated, and allowed a 1.5 hour recovery prior to dechorionation. Total RNA was isolated using Trizol (Invitrogen).
Label biotin
Label protocol Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description Wild type (w1118) embryos
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Jun 08, 2005
Last update date Jun 08, 2005
Contact name John M Abrams
E-mail(s) John.Abrams@UTSouthwestern.edu
Phone 214-648-9226
Fax 214-648-8694
Organization name University of Texas Southwestern Medical Center
Department Cell Biology
Lab Abrams
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390-9039
Country USA
 
Platform ID GPL72
Series (1)
GSE2780 Radiation-induced Dmp53-dependent expression profile

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
141200_at 125.1 P 0.003159
141201_at 43 A 0.364945
141202_at 886.2 P 0.000613
141203_at 186.1 P 0.002607
141204_at 832.3 P 0.000613
141205_at 330.1 P 0.001432
141206_at 1076.3 P 0.000491
141207_at 147.3 P 0.000613
141208_at 925 P 0.000491
141209_at 721.3 P 0.000944
141210_at 126.5 P 0.001432
141211_at 365.9 P 0.000491
141212_at 999.4 P 0.000491
141213_at 10.6 A 0.235169
141214_at 1244.7 P 0.000491
141215_at 963.6 P 0.002143
141216_at 586.6 P 0.000491
141217_at 456.7 P 0.000491
141218_at 2643.1 P 0.000491
141219_at 328.1 P 0.002143

Total number of rows: 14010

Table truncated, full table size 358 Kbytes.




Supplementary file Size Download File type/resource
GSM60480.CEL.gz 2.8 Mb (ftp)(http) CEL
GSM60480.EXP.gz 481 b (ftp)(http) EXP

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