|
Status |
Public on Jun 06, 2006 |
Title |
WT (w1118) control replicate 2 |
Sample type |
RNA |
|
|
Source name |
embryos, WT (w1118), control
|
Organism |
Drosophila melanogaster |
Characteristics |
Wild type (w1118), embryos 4-6.5 hours after egg laying, control
|
Extracted molecule |
total RNA |
Extraction protocol |
Approximately 1 gram of wild-type (w1118 strain) embryos was collected 0-2.5 hours after egg laying. The embryos were aged for 2.5 hours at 25C, mock-treated, and allowed a 1.5 hour recovery prior to dechorionation. Total RNA was isolated using Trizol (Invitrogen).
|
Label |
biotin
|
Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Wild type (w1118) embryos
|
Data processing |
Affymetrix Microarray Suite version 5.0
|
|
|
Submission date |
Jun 08, 2005 |
Last update date |
Jun 08, 2005 |
Contact name |
John M Abrams |
E-mail(s) |
John.Abrams@UTSouthwestern.edu
|
Phone |
214-648-9226
|
Fax |
214-648-8694
|
Organization name |
University of Texas Southwestern Medical Center
|
Department |
Cell Biology
|
Lab |
Abrams
|
Street address |
5323 Harry Hines Blvd
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390-9039 |
Country |
USA |
|
|
Platform ID |
GPL72 |
Series (1) |
GSE2780 |
Radiation-induced Dmp53-dependent expression profile |
|