|
| Status |
Public on Mar 30, 2011 |
| Title |
mitf-BRAFV600E;p53-/- melanoma 1 |
| Sample type |
RNA |
| |
|
| Source name |
Melanoma BRAFV600E;p53-/-
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: mitf-BRAFV600E;p53-/-
tissue: Melanoma
|
| Growth protocol |
Zebrafish of the indicated genotype were grown to 4-8 months of age, when a visible melanoma could be identified. The melanoma, and immediately adjacent skin was excised from the fish. For WT samples, the lateral length of the zebrafish skin was excised.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen, USA).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
Hybridization was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
|
| Scan protocol |
Scanning was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
|
| Description |
This sample is a BRAF;p53 melanoma
|
| Data processing |
Raw data (*.cel files) was normalized using GC-RMA followed by per gene and per chip normalization
|
| |
|
| Submission date |
Oct 05, 2010 |
| Last update date |
Mar 30, 2011 |
| Contact name |
Richard Mark White |
| E-mail(s) |
whiter@mskcc.org
|
| Phone |
646-888-3415
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Cancer Biology & Genetics
|
| Lab |
White Lab
|
| Street address |
1275 York Avenue MB 424
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE24528 |
Expression analysis of zebrafish melanoma and skin from the mitf-BRAFV600E;p53-/- line |
|
