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Status |
Public on Mar 30, 2011 |
Title |
BRAF;p53 2 |
Sample type |
RNA |
|
|
Source name |
tg(mitf-BRAFV600E);p53-/- embryos 2
|
Organism |
Danio rerio |
Characteristics |
genotype/variation: tg(mitf-BRAFV600e);p53-M214K-/- developmental stage: 80hpf tissue: embryo
|
Growth protocol |
Zebrafish of the indicated genotype were incrossed to produce embryos, which were grown to 80hpf.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen, USA).
|
Label |
Cy3
|
Label protocol |
Labeling was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
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|
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Hybridization protocol |
Hybridization was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
|
Scan protocol |
Scanning was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
|
Description |
This sample is an 80hpf transgenic zebrafish carrying a stable homozygous allele of mitf-BRAFV600E in the background of a p53-/- loss of function
|
Data processing |
Raw data (*.cel files) was normalized using GC-RMA followed by per gene and per chip normalization
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Submission date |
Oct 05, 2010 |
Last update date |
Mar 30, 2011 |
Contact name |
Richard Mark White |
E-mail(s) |
whiter@mskcc.org
|
Phone |
646-888-3415
|
Organization name |
Memorial Sloan Kettering Cancer Center
|
Department |
Cancer Biology & Genetics
|
Lab |
White Lab
|
Street address |
1275 York Avenue MB 424
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE24529 |
Expression analysis of transgenic embryos carrying the mitf-BRAFV600E allele in the presence or absence of p53 function |
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