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Sample GSM604815 Query DataSets for GSM604815
Status Public on Mar 30, 2011
Title BRAF;p53 2
Sample type RNA
 
Source name tg(mitf-BRAFV600E);p53-/- embryos 2
Organism Danio rerio
Characteristics genotype/variation: tg(mitf-BRAFV600e);p53-M214K-/-
developmental stage: 80hpf
tissue: embryo
Growth protocol Zebrafish of the indicated genotype were incrossed to produce embryos, which were grown to 80hpf.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol (Invitrogen, USA).
Label Cy3
Label protocol Labeling was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
 
Hybridization protocol Hybridization was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
Scan protocol Scanning was performed by the Genomics Core at Children's Hospital Boston according to standard Affymetrix protocols
Description This sample is an 80hpf transgenic zebrafish carrying a stable homozygous allele of mitf-BRAFV600E in the background of a p53-/- loss of function
Data processing Raw data (*.cel files) was normalized using GC-RMA followed by per gene and per chip normalization
 
Submission date Oct 05, 2010
Last update date Mar 30, 2011
Contact name Richard Mark White
E-mail(s) whiter@mskcc.org
Phone 646-888-3415
Organization name Memorial Sloan Kettering Cancer Center
Department Cancer Biology & Genetics
Lab White Lab
Street address 1275 York Avenue MB 424
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL1319
Series (1)
GSE24529 Expression analysis of transgenic embryos carrying the mitf-BRAFV600E allele in the presence or absence of p53 function

Data table header descriptions
ID_REF
VALUE GCRMA-normalized, averaged gene-level signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at -0.35762355
AFFX-BioB-5_at -0.08194914
AFFX-BioB-M_at -0.10100549
AFFX-BioC-3_at -0.021803914
AFFX-BioC-5_at -0.13122557
AFFX-BioDn-3_at -0.14032361
AFFX-BioDn-5_at -0.13339569
AFFX-CreX-3_at -0.10778379
AFFX-CreX-5_at -0.22458273
AFFX-DapX-3_at -1.2025851
AFFX-DapX-5_at -0.5390801
AFFX-DapX-M_at -0.94212836
AFFX-Dr-AB076373-1_at -1.5036219
AFFX-Dr-acta1-3_at -0.007737574
AFFX-Dr-acta1-5_at 0.24647184
AFFX-Dr-acta1-5_x_at 0.01309142
AFFX-Dr-acta1-M_at 0.006805272
AFFX-Dr-AF292559-1_at -1.8844063
AFFX-Dr-AF292559-2_s_at -0.5087607
AFFX-Dr-AF292559-3_s_at -0.20771202

Total number of rows: 15617

Table truncated, full table size 433 Kbytes.




Supplementary file Size Download File type/resource
GSM604815.CEL.gz 2.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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