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Status
Public on Jun 06, 2006
Title
Mutant (Dmp53NS) control replicate 1
Sample type
RNA
Source name
embryos, mutant (Dmp53NS), control
Organism
Drosophila melanogaster Characteristics
Mutant (Dmp53NS), embryos 4-6.5 hours after egg laying, control
Extracted molecule
total RNA
Extraction protocol
Approximately 1 gram of mutant (Dmp53NS strain) embryos was collected 0-2.5 hours after egg laying. The embryos were aged for 2.5 hours at 25C, mock-treated, and allowed a 1.5 hour recovery prior to dechorionation. Total RNA was isolated using Trizol (Invitrogen).
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Mutant (Dmp53NS) embryos
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Jun 08, 2005
Last update date
Jun 08, 2005
Contact name
John M Abrams
E-mail(s)
John.Abrams@UTSouthwestern.edu Phone
214-648-9226
Fax
214-648-8694
Organization name
University of Texas Southwestern Medical Center
Department
Cell Biology
Lab
Abrams
Street address
5323 Harry Hines Blvd
City
Dallas
State/province
TX
ZIP/Postal code
75390-9039
Country
USA
Platform ID
GPL72 Series (1)
GSE2780 Radiation-induced Dmp53-dependent expression profile
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 141200_at
214
P
0.000491
141201_at
15.1
A
0.834732
141202_at
706.2
P
0.000491
141203_at
992
P
0.000613
141204_at
299.4
P
0.000944
141205_at
655.6
P
0.000491
141206_at
484.1
P
0.000491
141207_at
1079.6
P
0.000491
141208_at
1150.6
P
0.000491
141209_at
1858.3
P
0.000491
141210_at
89.6
P
0.002607
141211_at
456.5
P
0.000491
141212_at
989.8
P
0.000491
141213_at
4.8
A
0.437646
141214_at
638.6
P
0.000491
141215_at
508.7
P
0.004591
141216_at
141.7
P
0.002607
141217_at
587.7
P
0.000613
141218_at
5365.4
P
0.000491
141219_at
360.8
P
0.002607
14010 357 Kbytes .Supplementary file Size Download File type/resource GSM60484.CEL.gz
2.7 Mb
(ftp) (http) CEL
GSM60484.EXP.gz
475 b
(ftp) (http) EXP
