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Status
Public on Oct 08, 2010
Title
DC.pDC.8-.Sp#3
Sample type
RNA
Source name
spleen
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
gentle teasing with tweezers, Blendzyme Liberase III and DNAse I digestion, depletion of CD3e, CD19, NK1.1 and TER119 positive cells using MACS system
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.pDC.8-.Sp#3, spleen CD8- plasmacytoid dentritic cells
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
Jun 07, 2018
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE74316 Reanalyzed by
GSE115450
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
24.2092
10344622
221.5866
10344624
500.6128
10344633
724.3703
10344637
349.9875
10344653
15.9181
10344658
245.7956
10344674
15.7823
10344679
42.3575
10344707
318.8463
10344713
268.3678
10344723
154.3975
10344725
37.2308
10344741
2641.8965
10344743
24.6589
10344750
718.5045
10344772
50.6549
10344789
220.5607
10344797
246.1172
10344799
285.7855
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605845_EA07068_105314_MoGene_DC.PDC.8-.SP#3.CEL.gz
4.3 Mb
(ftp) (http) CEL
Processed data included within Sample table
