|
Status |
Public on Feb 01, 2011 |
Title |
MET2 |
Sample type |
RNA |
|
|
Source name |
metastatic sarcoma
|
Organism |
Canis lupus familiaris |
Characteristics |
tissue type: Primary tumor disease group: metastatic
|
Treatment protocol |
Untreated tumors
|
Extracted molecule |
total RNA |
Extraction protocol |
Rneasy mini-kit from Qiagen
|
Label |
biotin
|
Label protocol |
Affymetrix 1-cycle 3' IVT labeling kit
|
|
|
Hybridization protocol |
Following fragmentation, 2 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Canine GeneChip version 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned with GeneChip Scanner 3000
|
Data processing |
Data were analyzed with JMPGenomics software (SAS). Normalization was performed with dChip.
|
|
|
Submission date |
Oct 08, 2010 |
Last update date |
Feb 01, 2011 |
Contact name |
Marlene Hauck |
E-mail(s) |
marlene_hauck@ncsu.edu
|
Organization name |
North Carolina State University
|
Department |
Clinical Sciences
|
Street address |
4700 Hillsborough Street
|
City |
Raleigh |
State/province |
NC |
ZIP/Postal code |
27606 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE24601 |
Metastatic and Nonmetastatic canine soft tissue sarcoma comparison |
|