|
Status |
Public on Apr 13, 2011 |
Title |
Control expression array-3 |
Sample type |
RNA |
|
|
Source name |
jejunum epithelium
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6-129 mixed genotype/variation: littermate controls tissue: jejunum epithelium
|
Treatment protocol |
mice were treated with tamoxifen for 5 consecutive days (1mg/day) and the jejunal epithelium was harvested 4 days after completion of the timecourse.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Trizol method and prepared for arrays using manufacturer's instructions (Affymetrix).
|
Label |
biotin
|
Label protocol |
according to manufacturer's instructions (Affymetrix)
|
|
|
Hybridization protocol |
according to manufacturer's instructions (Affymetrix)
|
Scan protocol |
according to manufacturer's instructions (Affymetrix)
|
Description |
mice were treated with tamoxifen for 5 consecutive days (1mg/day) and the jejunal epithelium was harvested 4 days after completion of the timecourse. RNA was extracted using the Trizol method and prepared for arrays using manufacturer's instructions (Affymetrix). Affymetrix mouse 4302 microarray
|
Data processing |
Microarray expression: RMA (bioconductor) with custom CDF (Mouse4302_Mm_REFSEQ) for background elimination and normalization. Limma for detection of differentially expressed genes between controls and Cdx2 KO or Cdx2-Cdx1 D KO.
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|
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Submission date |
Oct 12, 2010 |
Last update date |
Apr 14, 2011 |
Contact name |
Michael P Verzi |
Organization name |
Rutgers, the State University of New Jersey
|
Department |
Genetics
|
Lab |
Verzi
|
Street address |
145 Bevier Road
|
City |
Piscataway |
State/province |
NJ |
ZIP/Postal code |
08854 |
Country |
USA |
|
|
Platform ID |
GPL11044 |
Series (1) |
GSE24633 |
Cdx2 transcription factor binding in intestinal villus and gene expression profiling in Cdx mutant mice |
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