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Sample GSM608266 Query DataSets for GSM608266
Status Public on Jul 27, 2011
Title control animal 2
Sample type RNA
 
Source name proximal tubular cells, untreated
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: kidney
cell type: proximal tubular cells
phenotype: type I diabetes
treatment: untreated
Treatment protocol Isolated proximal tubular cells from diabetic rats were treated with or without C-peptide for 2 hrs.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from rat proximal tubular cells stored in RNA later using Rneasy (QIAGEN, Valencia, CA, USA).
Label biotin
Label protocol Total RNA were amplified and converted to cDNA and labeled using the Whole Transcript (WT) Sense Target Labeling Protocol.
 
Hybridization protocol From non-degraded high-quality total RNA, we prepared and hybridized biotinylated complementary ssDNA to Rat Gene 1.0 ST Arrays, and then washed, stained and scanned slides using standard protocols (Affymetrix Inc., Santa Clara, CA).
Scan protocol We used a GeneChip Scanner 3000 and standard Affymetrix protocols (Affymetrix Inc., Santa Clara, CA).
Description P50_Control2
Data processing Preprocessing was performed in Affymetrix Expression Console (v. 1.1) using the following methods: Summarization: PLIER, Background Correction: PM-GCBG, Normalization: Global Median.
 
Submission date Oct 13, 2010
Last update date Jul 27, 2011
Contact name Emma Lindahl
E-mail(s) emmalindahl@gmail.com
Organization name Karolinska Institutet
Street address Scheeles väg 2
City Stockholm
ZIP/Postal code 17177
Country Sweden
 
Platform ID GPL6247
Series (1)
GSE24687 C-peptide regulates early transcription in rat proximal tubular cells

Data table header descriptions
ID_REF
VALUE Normalized signal

Data table
ID_REF VALUE
10700001 8519.8
10700002 261.68
10700003 3100.58
10700004 129.44
10700005 2163.99
10700006 9.1
10700007 9.97
10700008 3.39
10700009 481.05
10700010 17.57
10700011 40.49
10700012 23.31
10700013 4308.77
10700014 2965.14
10700015 728.3
10700016 8.36
10700017 127.24
10700018 4.75
10700019 7.56
10700020 9256.43

Total number of rows: 29215

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM608266.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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