|
| Status |
Public on Oct 22, 2010 |
| Title |
5 dpl laser captured CCP replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Rat caudal cerebellar peduncle
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
age: 12 weeks at 0 dpl (days post lesion)
tissue: caudal cerebellar peduncles
time: 5 dpl (days post lesion)
|
| Treatment protocol |
Rat caudal cerebellar peduncles (CCP) were demyelinated using 0.1 % ethidium bromide. The lesioned CCPs were selectively isolated from frozen brains using laser capture microdissections at 5, 14 and 28 days postlesion before RNA extraction and microarray analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| Label |
biotin
|
| Label protocol |
Standard Illumina hybridisation protocol
|
| |
|
| Hybridization protocol |
Standard Illumina scanning protocol
|
| Scan protocol |
biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| Data processing |
Data were filtered, removing any non expressed probes according to the detection p-value, then variance stabilization was applied and finally the data were normalised using quantile normalization.
|
| |
|
| Submission date |
Oct 20, 2010 |
| Last update date |
Sep 06, 2013 |
| Contact name |
Julien Bauer |
| E-mail(s) |
jb393@cam.ac.uk
|
| Organization name |
Cambridge University
|
| Street address |
Tennis court road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 1QP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE24821 |
Retinoid X receptor gamma signaling accelerates CNS remyelination |
|
| Relations |
| Reanalyzed by |
GSE50042 |
