|
| Status |
Public on Oct 01, 2013 |
| Title |
Wildtype islets (Sample b) |
| Sample type |
RNA |
| |
|
| Source name |
Isolated mouse pancreatic islets from wildtype mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
Sex: Male
age: 15 month
genotype: wildtype(WT)
|
| Treatment protocol |
Pancreatic islets were purified by collagenase digestion.
|
| Growth protocol |
Mice were kept on a 12-hour light/dark cycle, food ad lib
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by QIAGEN RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
One round amplification protocol for total RNA following Affymetrix’ specifications using T7 promoter random primers for cDNA synthesis, and Invitrogen SuperScript II reverse transcriptase (www.affymetrix.com, GeneChip Expression Analysis, Technicam Manual).
|
| |
|
| Hybridization protocol |
16hrs at 45° C with rotation (60rpm) as described by Affymetrix in their GeneChip Expression Analysis Technical Manual.
|
| Scan protocol |
Using Affymetrix’ GeneChip Scanner 3000 7G and default parameters described by the manufacturer in their GeneChip Expression Analysis Technical Manual.
|
| Data processing |
Partek GS, customized to Partek defaults, using all extended probesets mps option
probe group file: MoEx-1_0-st-v1.r2.pgf
meta-probeset file: MoEx-1_0-st-v1.na31.mm9.probeset_extended.mps
|
| |
|
| Submission date |
Oct 31, 2010 |
| Last update date |
Apr 04, 2014 |
| Contact name |
Nini Guo |
| E-mail(s) |
nguo@jhmi.edu
|
| Phone |
4109554721
|
| Fax |
4106142987
|
| Organization name |
Johns Hopkins University
|
| Street address |
725 North Wolfe St. PCTB605
|
| City |
Baltimore |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE25040 |
Short telomeres compromise β-cell signaling and survival |
|
