|
| Status |
Public on Nov 30, 2010 |
| Title |
MSC - 12 months of age (#2) |
| Sample type |
RNA |
| |
|
| Source name |
MSC from inguinal fat pad
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
age: 12 month
|
| Treatment protocol |
cells cultured up to the 20th passage
|
| Growth protocol |
DMEM Dulbecco’s Modified Eagle Medium (1X) liquid (High Glucose) [with GlutaMAX 4500 mg/L D-Glucose without Sodium Pyruvate]
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extracted using QIAGEN Rneasy kit, following the manifacturer's instruction
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis followed by Cy3-labeling
|
| |
|
| Hybridization protocol |
Cy3-labeled cDNA was hybridized overnight at 42°C to mus musculus 12x135K Array (Roche NimbleGen)
|
| Scan protocol |
arrays were scanned with GenePix 4400A Microarray Scanner
|
| Description |
cells cultured at 20th passage
|
| Data processing |
the data were extracted from scanned images using NimbleScan software
|
| |
|
| Submission date |
Nov 01, 2010 |
| Last update date |
Nov 29, 2010 |
| Contact name |
Mirco Galiè |
| E-mail(s) |
mirco.galie@univr.it
|
| Organization name |
University of Verona
|
| Department |
Neuroscience, Biomedicine and Movement Sciences
|
| Lab |
Anatomy and Histology
|
| Street address |
via Le Grazie, 8
|
| City |
Verona |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10192 |
| Series (2) |
| GSE25069 |
Whole-genome microarray of adipose derived mesenchymal stem cells from differentially-aged mice |
| GSE25685 |
Profiling of mesenchymal stem cells from adipose tissue of differentially aged mice |
|
