|
| Status |
Public on Sep 07, 2011 |
| Title |
HB008AK_2/22/08_11:54_AM |
| Sample type |
RNA |
| |
|
| Source name |
colorectal cancer tissue
|
| Organism |
Homo sapiens |
| Characteristics |
age at diagnosis: 83
gender: male
tumor stage: III
localization: colon
microsatellite instability (msi) status: MSI-L
tissue type: primary tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Primary tumor: AllPrep DNA/RNA Mini Kit (Qiagen); normal colonic mucosa: Ambion RiboPure Kit (Applied Biosystems)
|
| Label |
digoxigenein (DIG)-labeled
|
| Label protocol |
NanoAmp RT-IVT Labeling Kit from Applied Biosystems and DIG-UTP from Roche
|
| |
|
| Hybridization protocol |
Chemilunimescent Detection Kit from Applied Biosystems
|
| Scan protocol |
Scanned using AB1700 Chemilunimescent Microarray Analyzer
|
| Description |
A53_PT
|
| Data processing |
Image preparation, signal intensity quantification and initial analysis were performed utsing the accompanying software from Applied biosystems (version 1.1.1). Only probes with normalized signals more than three standard deviations greater than the local background levels in at least half of the samples were included. Values with low quality scores were noted as missing and probes affected by array batch variation were identified and removed. The resulting dataset included 17 085 probes.
|
| |
|
| Submission date |
Nov 01, 2010 |
| Last update date |
Sep 07, 2011 |
| Contact name |
Trude Holmeide Ågesen |
| E-mail(s) |
trude.holmeide.aagesen@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Cancer Prevention
|
| Street address |
Ullernch. 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE25071 |
Gene expression comparison of colorectal cancer samples from patients diagnosed at a young and elderly age |
|
