|
| Status |
Public on Aug 30, 2013 |
| Title |
P53 knockout at E12.5, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
P53-/-
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblasts
strain: C57BL/6J
genotype/variation: P53-/-
age: E12.5
|
| Growth protocol |
Fibroblasts isolated from day 9.0 old mouse embryos for the Mta1-/- and 12.5 days old for the P53-/- mice. The strain of the mice used was C57BL/6J.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol was used for the RNA extraction, manufacturer's instructions were followed
|
| Label |
biotin
|
| Label protocol |
rRNA reduced samples were amplified and labelled using Genechip WT cDNA Synthesis/Amplification Kit and GeneChip WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA)
|
| |
|
| Hybridization protocol |
Samples were processed using the Affymetrix hybridization, wash and stain kits. 200µl of the hybridization mixture was hybridized onto each Mouse Exon 1.0 st array. Hybridized at 45°C with 60rpm rotation for 17 hours in Affymetrix hybridization oven 640
|
| Scan protocol |
Affymetrix Genechip scanner 3000 7G
|
| Description |
mouse embryonic fibroblasts from P53 knockout mice
|
| Data processing |
Data were processed using Genespring GX 10.0.2 (Affymetrix Technologies,Inc) . The raw data was summerized using RMA16 algorithm which is inbuilt in Genespring GX,followed by the log transformation, baseline transformation and finally quantile normalization.
Normalized gene level expression values from Genespring software (Affymetrix)
|
| |
|
| Submission date |
Nov 01, 2010 |
| Last update date |
Aug 30, 2013 |
| Contact name |
krishna ghanta |
| Organization name |
GWU
|
| Street address |
2300 eye st NW
|
| City |
washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (1) |
| GSE25072 |
Expression profiling data of Mta1 from E9.0 mouse embryonic fibroblasts |
|
