|
| Status |
Public on May 20, 2022 |
| Title |
FUS H517Q homozygous (FUS_hom_1) |
| Sample type |
SRA |
| |
|
| Source name |
iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: iPSC
cell type: iPSC-derived motor neuron
disease state: ALS
treatment: motor neuron differentiation
time: Day 30
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using microRNeasy mini kit (Qiagen).
1.0 ug of total RNA was used to construct sequencing libraries using the NEBNext Small RNA kit
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using the FastX-toolkit
Trimmed sequence reads were mapped to hg19 using bowtie
Read counts were estimated using R package feature count and normalization were performed using DESeq2
Assembly: hg19
Supplementary files format and content: tab-delimited text file includes raw readcounts per microRNA for each Sample
|
| |
|
| Submission date |
May 17, 2022 |
| Last update date |
May 24, 2022 |
| Contact name |
Akshay Bhinge |
| E-mail(s) |
drabhinge@gmail.com
|
| Organization name |
Living Systems Institute, University of Exeter
|
| Department |
Medical School
|
| Street address |
Stocker Road
|
| City |
Exeter |
| ZIP/Postal code |
EX4 4QD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL15520 |
| Series (2) |
| GSE203170 |
Small RNA-sequencing of FUS H517Q and healthy iPSC-derived motor neurons |
| GSE203173 |
FUS H517Q and healthy iPSC-derived motor neurons |
|
| Relations |
| BioSample |
SAMN28488259 |
| SRA |
SRX15302721 |
