|
| Status |
Public on May 20, 2022 |
| Title |
shFLG IL-4/IL-13 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Spontaneously immortalized human keratinocyte cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HaCaT
knockdown: shFLG
treatment: IL-4/IL-13
treatment compound concentration: 50 ng/ml / 50 ng/ml
|
| Treatment protocol |
shC and shFLG HaCaTs were cultured to 80% confluence and then treated with the indicated compounds and incubated for 24 h at 37°C, 5% CO2. Following the incubation total RNA was isolated from the cells.
|
| Growth protocol |
Filaggrin-sufficient (shC) and filaggrin-insufficient (shFLG) HaCaT keratinocytes were cultured at 37°C, 5% CO2 in DMEM supplemented with 10% FCS, 2 mM L-glutamine, 100 U/ml penicilin and 100 ug/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini kit (Qiagen) according to manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Austin, TX, USA) according to the manufacturer's protocol
|
| |
|
| Hybridization protocol |
Hybridization and washing were performed according to the Illumina Manual 'Direct Hybridization Assay Guide'
|
| Scan protocol |
Scanning was performed on the Illumina iScan (Illumina, Inc., San Diego, CA, USA) according to the Illumina protocol 'Whole-Genome Gene Expression Direct Hybridization Assay'
|
| Description |
Sample 29
|
| Data processing |
Image analysis and extraction of raw expression data was performed with Illumina GenomeStudio v2011.1 Gene Expression software with default settings
|
| |
|
| Submission date |
May 19, 2022 |
| Last update date |
May 20, 2022 |
| Contact name |
Anna Supernat |
| E-mail(s) |
anna.supernat@gumed.edu.pl
|
| Organization name |
Medical University of Gdansk
|
| Department |
Division of Translational Oncology
|
| Street address |
Dębinki 1
|
| City |
Gdańsk |
| ZIP/Postal code |
80-211 |
| Country |
Poland |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE203409 |
Gene expression profiling of filaggrin-insufficient keratinocytes exposed to inflammatory mediators and allergens |
|
