|
| Status |
Public on Nov 18, 2010 |
| Title |
4 PPARg +/-LysCre IL-4 macrophage+RSG |
| Sample type |
RNA |
| |
|
| Source name |
PPARg +/-LysCre bone marrow-derived macrophage treated with IL-4+RSG
|
| Organism |
Mus musculus |
| Characteristics |
mouse: 4
strain: C57Bl/6
genotype/variation: PPARg +/-LysCre
cell type: bone marrow-derived alternatively activated macrophage
treatment: Rosiglitazone
time: 9 days
|
| Treatment protocol |
Cell were treated after plating into 6-well cell culture plates with the indicated cytokines and vehicle (DMSO:ethanol) or 1 uM Rosiglitazone.
|
| Growth protocol |
Bone marrow cells were isolated from the femur, washed twice with PBS and cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin and 20 ng/ml M-CSF+20 ng/ml IL-4 for macrophage or 20 ng/ml GM-CSF+20 ng/ml IL-4 for iDC differentiation. Fresh media and cytokines were added every other day for 9 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
DIG
|
| Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
|
| |
|
| Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
|
| Description |
Gene expression data of IL-4+RSG-treated bone marrow-derived macrophages derived from PPARg +/-LysCre mouse
Data Sample 14.txt
|
| Data processing |
The data were generated with ABI1700 and txt files were further analyzed in GeneSpring 7.3.
|
| |
|
| Submission date |
Nov 03, 2010 |
| Last update date |
Nov 18, 2010 |
| Contact name |
Attila Szanto |
| E-mail(s) |
attila.szanto@gmail.com
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Lee Lab
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02446 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (2) |
| GSE16387 |
Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation |
| GSE25125 |
PPARg and IL-4-induced gene expression data from PPARg +/- LysCre and PPARg fl/- LysCre mouse bone marrow-derived alternatively activated macrophages and immature dendritic cells (iDCs) |
|
