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Sample GSM617251 Query DataSets for GSM617251
Status Public on Nov 18, 2010
Title 8 PPARg fl/-LysCre iDC
Sample type RNA
 
Source name PPARg fl/-LysCre bone marrow-derived iDC
Organism Mus musculus
Characteristics mouse: 8
strain: C57Bl/6
genotype/variation: PPARg fl/-LysCre
cell type: bone marrow-derived immature dendritic cell
treatment: Control
time: 9 days
Treatment protocol Cell were treated after plating into 6-well cell culture plates with the indicated cytokines and vehicle (DMSO:ethanol) or 1 uM Rosiglitazone.
Growth protocol Bone marrow cells were isolated from the femur, washed twice with PBS and cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin and 20 ng/ml M-CSF+20 ng/ml IL-4 for macrophage or 20 ng/ml GM-CSF+20 ng/ml IL-4 for iDC differentiation. Fresh media and cytokines were added every other day for 9 days.
Extracted molecule total RNA
Extraction protocol Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
Label DIG
Label protocol 1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
 
Hybridization protocol Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
Scan protocol Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
Description Gene expression data of bone marrow-derived iDC derived from PPARg fl/-LysCre mouse
Data Sample 31.txt
Data processing The data were generated with ABI1700 and txt files were further analyzed in GeneSpring 7.3.
 
Submission date Nov 03, 2010
Last update date Nov 18, 2010
Contact name Attila Szanto
E-mail(s) attila.szanto@gmail.com
Organization name Massachusetts General Hospital
Department Molecular Biology
Lab Lee Lab
Street address 185 Cambridge St.
City Boston
State/province MA
ZIP/Postal code 02446
Country USA
 
Platform ID GPL2995
Series (2)
GSE16387 Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation
GSE25125 PPARg and IL-4-induced gene expression data from PPARg +/- LysCre and PPARg fl/- LysCre mouse bone marrow-derived alternatively activated macrophages and immature dendritic cells (iDCs)

Data table header descriptions
ID_REF
VALUE Normalized signal intensity after per chip normalization and per gene normalization.

Data table
ID_REF VALUE
370264 1.1984233
577485 0.62508494
456277 2.2437856
422129 0.8566073
312133 2.3500557
828031 0.7752935
624353 0.9884799
774404 1.0030398
301015 1.5395873
371879 0.9935505
388961 0.5597116
892920 0.64488965
498201 1.2364554
388093 0.63635653
787300 1.0088127
470796 1.0403079
437610 0.34963813
466290 1.4165895
329956 0.7992907
397784 0.50427485

Total number of rows: 33012

Table truncated, full table size 552 Kbytes.




Supplementary file Size Download File type/resource
GSM617251.txt.gz 887.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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