|
| Status |
Public on Nov 01, 2011 |
| Title |
lox/loxFO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lox/loxFO_rep1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6, possibly with some contribution of CBA
tissue: spleen
cell type: follicular (FO) B cells
genotype/variation: CD19-Creki/+Dicerfl/fl
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of purified populations was extracted with TRIzol (Invitrogen) following manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Amount of nucleic acid labeled: 120ng. Commercial miRNA Microarray System with miRNA Complete Labeling and Hyb Kit by following manufacturer instructions. Agilent manual G4170-90011 Ver. 2.0 June 2008. Fluorescent Cyanine 3-pCp molecules are ligated with T4 RNA ligase to dephosphorilated 3' ends from the RNA sample.
|
| |
|
| Hybridization protocol |
Chamber type: SureHyb hybridization chamber (Agilent). Quantity of each labeled extract used: all the labeled sample. Duration: 40 hours. Volume: 45 uL. Temperature (ºC): 55.
|
| Scan protocol |
Scanned on an G2565C DNA microarray scanner (Agilent). Images were quantified using Agilent Feature Extraction Software (ver. 10.1.1).
|
| Description |
US22502553_251911910078_S01_miRNA-v1_95_May07_1_3.txt
|
| Data processing |
Images were quantified and normalized using default normalization with Agilent Feature Extraction Software (ver. 10.1.1).
|
| |
|
| Submission date |
Nov 04, 2010 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Almudena Ramiro |
| E-mail(s) |
arodriguezr@cnio.es
|
| Organization name |
CNIO
|
| Street address |
Calle Melchor Fernandez Almagro 3
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE25139 |
miRNA expression profile of follicular (FO) and marginal zone (MZ) B cells |
|
