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Sample GSM61802 Query DataSets for GSM61802
Status Public on Jul 23, 2005
Title Cell Culture Exposure (3MAC Halo2)
Sample type RNA
 
Channel 1
Source name Pooled Control
Organism Rattus norvegicus
Characteristics Cy3
Biomaterial provider Agilent
Treatment protocol Agilent
Growth protocol Agilent
Extracted molecule total RNA
Extraction protocol Agilent
Label Cy3
Label protocol Agilent
 
Channel 2
Source name 3MAC Halo2
Organism Rattus norvegicus
Characteristics Cy5
Biomaterial provider Agilent
Treatment protocol Agilent
Growth protocol Agilent
Extracted molecule total RNA
Extraction protocol Agilent
Label Cy5
Label protocol Agilent
 
 
Hybridization protocol Agilent
Scan protocol Agilent
Description Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
Data processing GeneSpring7.0 and Partek Pro.
 
Submission date Jun 17, 2005
Last update date Oct 28, 2005
Contact name Roderic Eckenhoff
E-mail(s) roderic.eckenhoff@uphs.upenn.edu
Phone 215-662-3705
Organization name University of Pennsylvania
Department
Street address
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL890
Series (1)
GSE2982 Effect of inhaled anesthetic to cultured cortical neurons

Data table header descriptions
ID_REF
VALUE LogRatio
CH1_SIG_MEAN gProcessedSignal
CH2_SIG_MEAN rProcessedSignal
CH1_BKG_MEAN gBGMeanSignal
CH2_BKG_MEAN rBGMeanSignal

Data table
ID_REF VALUE CH1_SIG_MEAN CH2_SIG_MEAN CH1_BKG_MEAN CH2_BKG_MEAN
1 -2.00E+00 2.73E+04 7.29E+01 3.00E+01 4.27E+01
2 0.00E+00 2.90E+01 5.80E+01 3.00E+01 4.33E+01
3 -2.28E-03 6.63E+01 6.59E+01 3.05E+01 4.29E+01
4 -5.33E-03 5.47E+03 5.41E+03 3.05E+01 4.33E+01
5 3.15E-01 7.05E+01 1.46E+02 3.04E+01 4.31E+01
6 -1.34E-01 2.56E+02 1.88E+02 3.02E+01 4.30E+01
7 -2.00E+00 2.74E+04 5.54E+01 3.08E+01 4.36E+01
8 -4.40E-02 6.37E+02 5.76E+02 3.07E+01 4.29E+01
9 1.82E-01 4.67E+01 7.10E+01 3.06E+01 4.31E+01
10 -4.57E-02 4.21E+03 3.79E+03 3.09E+01 4.26E+01
11 1.37E-01 6.26E+01 8.58E+01 3.09E+01 4.26E+01
12 -6.88E-02 2.55E+02 2.18E+02 3.10E+01 4.27E+01
13 -3.44E-02 2.00E+03 1.84E+03 3.06E+01 4.23E+01
14 -2.00E+00 2.93E+04 3.87E+01 3.08E+01 4.24E+01
15 -1.41E-01 1.73E+04 1.25E+04 3.07E+01 4.27E+01
16 -3.10E-02 3.03E+02 2.82E+02 3.14E+01 4.34E+01
17 8.02E-02 2.22E+02 2.67E+02 3.10E+01 4.26E+01
18 0.00E+00 4.14E+01 5.09E+01 3.13E+01 4.30E+01
19 -4.74E-02 4.75E+02 4.26E+02 3.07E+01 4.30E+01
20 -7.64E-03 5.36E+01 5.26E+01 3.12E+01 4.23E+01

Total number of rows: 21575

Table truncated, full table size 1074 Kbytes.




Supplementary data files not provided

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