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Status
Public on Jul 23, 2005
Title
Cell Culture Exposure (3MAC Halo2)
Sample type
RNA
Channel 1
Source name
Pooled Control
Organism
Rattus norvegicus
Characteristics
Cy3
Biomaterial provider
Agilent
Treatment protocol
Agilent
Growth protocol
Agilent
Extracted molecule
total RNA
Extraction protocol
Agilent
Label
Cy3
Label protocol
Agilent
Channel 2
Source name
3MAC Halo2
Organism
Rattus norvegicus
Characteristics
Cy5
Biomaterial provider
Agilent
Treatment protocol
Agilent
Growth protocol
Agilent
Extracted molecule
total RNA
Extraction protocol
Agilent
Label
Cy5
Label protocol
Agilent
Hybridization protocol
Agilent
Scan protocol
Agilent
Description
Fetal brains were obtained from 3 pregnant rats at pregnant day 17. Primary cortical neurons were isolated and pooled separately from each mother and plated onto 100mm ´ dishes, for a total of 4 plates from each pregnant rat. At Day in Vitro (DIV) 16, 3 of the 4 platesfrom the same mother, were exposed to either 1 or 3 MAC halothane (about 1% or 3%) or 3 MAC isoflurane (about 4%) in a gas tight chamber inside the culture incubator for 6 hr. The 4th plate from each mother treated with air were used (inside the same incubator, but outside the chamber) as controls. Isoflurane at 1 MAC was not conducted because of the complete lack of transcriptional response in the animals exposed to 1 MAC of isoflurane. The cells were recovered for an additional 6 hr, harvested, frozen by liquid nitrogen, and stored at –80oC.
Data processing
GeneSpring7.0 and Partek Pro.
Submission date
Jun 17, 2005
Last update date
Oct 28, 2005
Contact name
Roderic Eckenhoff
E-mail(s)
roderic.eckenhoff@uphs.upenn.edu
Phone
215-662-3705
Organization name
University of Pennsylvania
Department
Street address
City
Philadelphia
State/province
PA
ZIP/Postal code
19104
Country
USA
Platform ID
GPL890
Series (1)
GSE2982
Effect of inhaled anesthetic to cultured cortical neurons
Data table header descriptions
ID_REF
VALUE
LogRatio
CH1_SIG_MEAN
gProcessedSignal
CH2_SIG_MEAN
rProcessedSignal
CH1_BKG_MEAN
gBGMeanSignal
CH2_BKG_MEAN
rBGMeanSignal
Data table
ID_REF
VALUE
CH1_SIG_MEAN
CH2_SIG_MEAN
CH1_BKG_MEAN
CH2_BKG_MEAN
1
-2.00E+00
2.73E+04
7.29E+01
3.00E+01
4.27E+01
2
0.00E+00
2.90E+01
5.80E+01
3.00E+01
4.33E+01
3
-2.28E-03
6.63E+01
6.59E+01
3.05E+01
4.29E+01
4
-5.33E-03
5.47E+03
5.41E+03
3.05E+01
4.33E+01
5
3.15E-01
7.05E+01
1.46E+02
3.04E+01
4.31E+01
6
-1.34E-01
2.56E+02
1.88E+02
3.02E+01
4.30E+01
7
-2.00E+00
2.74E+04
5.54E+01
3.08E+01
4.36E+01
8
-4.40E-02
6.37E+02
5.76E+02
3.07E+01
4.29E+01
9
1.82E-01
4.67E+01
7.10E+01
3.06E+01
4.31E+01
10
-4.57E-02
4.21E+03
3.79E+03
3.09E+01
4.26E+01
11
1.37E-01
6.26E+01
8.58E+01
3.09E+01
4.26E+01
12
-6.88E-02
2.55E+02
2.18E+02
3.10E+01
4.27E+01
13
-3.44E-02
2.00E+03
1.84E+03
3.06E+01
4.23E+01
14
-2.00E+00
2.93E+04
3.87E+01
3.08E+01
4.24E+01
15
-1.41E-01
1.73E+04
1.25E+04
3.07E+01
4.27E+01
16
-3.10E-02
3.03E+02
2.82E+02
3.14E+01
4.34E+01
17
8.02E-02
2.22E+02
2.67E+02
3.10E+01
4.26E+01
18
0.00E+00
4.14E+01
5.09E+01
3.13E+01
4.30E+01
19
-4.74E-02
4.75E+02
4.26E+02
3.07E+01
4.30E+01
20
-7.64E-03
5.36E+01
5.26E+01
3.12E+01
4.23E+01
Total number of rows: 21575 Table truncated, full table size 1074 Kbytes .Supplementary data files not provided