|
| Status |
Public on May 01, 2011 |
| Title |
#2691 HUH7 |
| Sample type |
RNA |
| |
|
| Source name |
Huh7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh7
cell type: Hepatocyte
permissiveness to hcv: Non-Permissive
|
| Growth protocol |
Cells were maintianed in DMEM (High Glucose) supplemented with 10% Fetal Calf Serum and 1% Pennicillin/Streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with GeneChip Operating Software, version 1.0.0.046 using Affymetrix default analysis settings (MAS 5.0 algorithm) and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Nov 05, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Bray Denard |
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Molecular Genetics
|
| Lab |
Jin Ye Lab
|
| Street address |
5323 Harry Hines Blvd. L5.226
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE25156 |
Host Factors with Reduced Expression in Two HCV Permissive Cell Lines as Compared to the Non-Permissive Parent Cell Line Huh7 |
|
