|
Status |
Public on Nov 12, 2010 |
Title |
IkkβΔ_replicate2 |
Sample type |
RNA |
|
|
Source name |
mature Ly-6G+ thioglycollate-elicited peritoneal neutrophils
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype/variation: IkkβΔ
|
Extracted molecule |
total RNA |
Extraction protocol |
Ly-6G+ neutrophils were collected and total RNA was extracted with TRIzol® (Invitrogen) and was purified by RNA micro cleanup Kit (Qiagen).
|
Label |
Biotin, Cy3
|
Label protocol |
Biotinylated cRNA was synthesized using an RNA Amplification Kit (Ambion) following manufacturer's directions.
|
|
|
Hybridization protocol |
Biotin-labeled cRNA was hybridized to Illumina Mouse-Ref8 BeadChips (Illumina) following manufacturer's directions.
|
Scan protocol |
Scan protocol was followed manufacturer's directions.
|
Description |
4432281011_B
|
Data processing |
The data were quantile normalized using BeadStudio v3.1 software. Data analysis and quality control were carried out using Partek® software.
|
|
|
Submission date |
Nov 09, 2010 |
Last update date |
Nov 12, 2010 |
Contact name |
Liang-Chuan Lai |
E-mail(s) |
llai@ntu.edu.tw
|
Phone |
886-2-23123456
|
Organization name |
National Taiwan University
|
Department |
Physiology
|
Street address |
1 Jen-Ai Road, Sec 1
|
City |
Taipei |
State/province |
Taiwan |
ZIP/Postal code |
10051 |
Country |
Taiwan |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE25211 |
IL-1β-driven neutrophil biogenesis and survival preserve anti-bacterial defense in IKKβ deficiency |
|