|
| Status |
Public on Nov 12, 2010 |
| Title |
IkkβF/F_replicate1 |
| Sample type |
RNA |
| |
|
| Source name |
mature Ly-6G+ thioglycollate-elicited peritoneal neutrophils
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: IkkβF/F
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ly-6G+ neutrophils were collected and total RNA was extracted with TRIzol® (Invitrogen) and was purified by RNA micro cleanup Kit (Qiagen).
|
| Label |
Biotin, Cy3
|
| Label protocol |
Biotinylated cRNA was synthesized using an RNA Amplification Kit (Ambion) following manufacturer's directions.
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was hybridized to Illumina Mouse-Ref8 BeadChips (Illumina) following manufacturer's directions.
|
| Scan protocol |
Scan protocol was followed manufacturer's directions.
|
| Description |
4432281011_E
|
| Data processing |
The data were quantile normalized using BeadStudio v3.1 software. Data analysis and quality control were carried out using Partek® software.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Nov 12, 2010 |
| Contact name |
Liang-Chuan Lai |
| E-mail(s) |
llai@ntu.edu.tw
|
| Phone |
886-2-23123456
|
| Organization name |
National Taiwan University
|
| Department |
Physiology
|
| Street address |
1 Jen-Ai Road, Sec 1
|
| City |
Taipei |
| State/province |
Taiwan |
| ZIP/Postal code |
10051 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE25211 |
IL-1β-driven neutrophil biogenesis and survival preserve anti-bacterial defense in IKKβ deficiency |
|
