|
| Status |
Public on Oct 31, 2012 |
| Title |
human bronchial epithelial cells from asthmatic donor 5 |
| Sample type |
RNA |
| |
|
| Source name |
Primary bronchial epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Primary bronchial epithelial cells
disease state: ashtma
|
| Treatment protocol |
(Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
HBECS were plated on transwells and grown at air-liquid interface
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization was perfomed according to manufacturers protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
miRNA expression data
|
| Data processing |
The data were analyzed with miRNA QC tool provided by Affymetrix.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Oct 31, 2012 |
| Contact name |
Melanie J. Jardim |
| E-mail(s) |
Jardim.Melanie@epa.gov
|
| Phone |
919-966-7320
|
| Fax |
919-966-6271
|
| Organization name |
U.S. EPA
|
| Department |
NHEERL
|
| Lab |
EPHD
|
| Street address |
104 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27514 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE25230 |
microRNA expression data in bronchial epithelial cells from healthy and asthmatic donors |
|
