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Sample GSM620995 Query DataSets for GSM620995
Status Public on Oct 31, 2012
Title human bronchial epithelial cells from asthmatic donor 7
Sample type RNA
 
Source name Primary bronchial epithelial cells
Organism Homo sapiens
Characteristics cell type: Primary bronchial epithelial cells
disease state: ashtma
Treatment protocol (Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0,7% NaCl, 0,04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL).
Growth protocol HBECS were plated on transwells and grown at air-liquid interface
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization was perfomed according to manufacturers protocol.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description miRNA expression data
Data processing The data were analyzed with miRNA QC tool provided by Affymetrix.
 
Submission date Nov 09, 2010
Last update date Oct 31, 2012
Contact name Melanie J. Jardim
E-mail(s) Jardim.Melanie@epa.gov
Phone 919-966-7320
Fax 919-966-6271
Organization name U.S. EPA
Department NHEERL
Lab EPHD
Street address 104 Mason Farm Road
City Chapel Hill
State/province NC
ZIP/Postal code 27514
Country USA
 
Platform ID GPL8786
Series (1)
GSE25230 microRNA expression data in bronchial epithelial cells from healthy and asthmatic donors

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
hsa-let-7a_st 313.6127
hsa-let-7a-star_st 0.6335204
hsa-let-7b_st 4565.427
hsa-let-7b-star_st 1.364133
hsa-let-7c_st 893.5123
hsa-let-7c-star_st 1.216531
hsa-let-7d_st 194.92
hsa-let-7d-star_st 0.8613586
hsa-let-7e_st 272.2105
hsa-let-7e-star_st 1.324049
hsa-let-7f_st 12.62737
hsa-let-7f-1-star_st 1.112387
hsa-let-7f-2-star_st 0.9441875
hsa-let-7g_st 2.605799
hsa-let-7g-star_st 1.487519
hsa-let-7i_st 11.22966
hsa-let-7i-star_st 0.9441875
hsa-miR-1_st 1.310579
hsa-miR-100_st 21.58183
hsa-miR-100-star_st 0.9038945

Total number of rows: 847

Table truncated, full table size 21 Kbytes.




Supplementary file Size Download File type/resource
GSM620995_Asthma_7.CEL.gz 136.4 Kb (ftp)(http) CEL
Processed data included within Sample table

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