|
| Status |
Public on Mar 01, 2011 |
| Title |
Muscle-FamilyHistoryNegative-8 |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
gender: male
age (years): 28
family history: Family history negative
body mass index (kg/m2): 24
fasting glucose (ogtt): 78.5
2 hour glucose (ogtt): 58
hemoglobin a1c: 4.7
fasting glucose (iv0gavg): 83
fasting insulin (iv0inavg): 1.7
si: 14.28
waist hip ratio: 0.865
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15ug of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix GeneChip Human Genome U133 Plus 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Jos3674
Gene expression of skeletal muscle from subject without diabetes
|
| Data processing |
Data were analyzed using Affymetrix GCOS v.1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
|
| |
|
| Submission date |
Nov 17, 2010 |
| Last update date |
Mar 01, 2011 |
| Contact name |
Mary Elizabeth Patti |
| E-mail(s) |
mary.elizabeth.patti@joslin.harvard.edu
|
| Phone |
6177351966
|
| Fax |
6177322593
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Research Division
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE25462 |
Increased SRF Transcriptional Activity is a Novel Signature of Insulin Resistance in Humans and Mice |
|
