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Sample GSM624964 Query DataSets for GSM624964
Status Public on Mar 01, 2011
Title Muscle-Diabetes-4
Sample type RNA
 
Source name skeletal muscle
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: male
age (years): 60
family history: DM
body mass index (kg/m2): 27
fasting glucose (ogtt): NA
2 hour glucose (ogtt): NA
hemoglobin a1c: 8.5
fasting glucose (iv0gavg): 119
fasting insulin (iv0inavg): 5.3
si: NA
waist hip ratio: 0.94
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol.
 
Hybridization protocol 15ug of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix GeneChip Human Genome U133 Plus 2.0 array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description Jos3746
Gene expression of skeletal muscle from subject with type 2 diabetes
Data processing Data were analyzed using Affymetrix GCOS v.1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
 
Submission date Nov 17, 2010
Last update date Mar 01, 2011
Contact name Mary Elizabeth Patti
E-mail(s) mary.elizabeth.patti@joslin.harvard.edu
Phone 6177351966
Fax 6177322593
Organization name Joslin Diabetes Center
Department Research Division
Street address 1 Joslin Place
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL570
Series (1)
GSE25462 Increased SRF Transcriptional Activity is a Novel Signature of Insulin Resistance in Humans and Mice

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1705.2
AFFX-BioB-M_at 1365.9
AFFX-BioB-3_at 941.2
AFFX-BioC-5_at 3843.8
AFFX-BioC-3_at 3458
AFFX-BioDn-5_at 10259.3
AFFX-BioDn-3_at 24202.3
AFFX-CreX-5_at 57792.6
AFFX-CreX-3_at 72974.7
AFFX-DapX-5_at 3752.5
AFFX-DapX-M_at 8144.5
AFFX-DapX-3_at 11521.9
AFFX-LysX-5_at 737.6
AFFX-LysX-M_at 1726.6
AFFX-LysX-3_at 2541.8
AFFX-PheX-5_at 726.4
AFFX-PheX-M_at 702.2
AFFX-PheX-3_at 854.7
AFFX-ThrX-5_at 1986.6
AFFX-ThrX-M_at 1940.1

Total number of rows: 54675

Table truncated, full table size 902 Kbytes.




Supplementary file Size Download File type/resource
GSM624964.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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